Detection amplicon size via primer?

More İbrahim Türkel's questions See All

Biofilm susceptibility testing?

Hi everyone i want determine antibiotic susceptibility of biofilm-state bacteria to compare with planktonic-state bacteria. i searched some papers and met some description like MBEC, "MBEC" is the...

01 February 2017 2,864 5 View

Which method is the best to study adaptive antibitoic resistance in gram negative bacteria?

Which method is the best to study adaptive antibitoic resistance in gram negative bacteria? How many days need to exposure bacteria with sub mic level of antibiotic?

08 September 2016 7,473 0 View

Can i use 2-ΔΔCt and 2-ΔCt formulas together for real time data analyze?

Since getting data from my real time pcr experiments, i'm struggling to analyze these datas. i have 100 bacteria strain isolated from different patients and i want to detect gene expression...

03 April 2016 3,046 11 View

Which method should i use for real time pcr?

Hi all i want to analyze real time pcr results but i have no experience at all. My question is i have Cq value of my gene in resitant strain and suspectibile strain. Also i normalized them with...

03 April 2016 6,907 16 View

Can intermediate (I) value of antimicrobial susceptibility refer as a resistant?

As you know, antibiogram resulted as susceptible (S), intermediate (I) or resistant(R). i also want to detect gene expression between resistant and susceptibel strains. Can i think intermediates...

03 April 2016 4,931 10 View

What must plant contains to have an antibacterial porperty?

Dear Reserachers i want to ask you about plant which have antibacterial. As you know, plants is going to take important point fighting against higly resistance bacteria, fungus etc. Also inhibits...

09 October 2015 8,115 6 View

Any tips for absolute quantification at real time pcr?

Hi all i am new at designing new method for real time pcr and i want to detect some gene expression at klebsiella pneumoniae. i am using 5 ng cDNA template for reaction. My question is which...

09 October 2015 4,125 7 View

Does anyone have experience about plasmid interaction between essential oils and plasmid DNA?

Hi all we want to test plants essential oil vis destilastion method and want to try interactions with plasmid. Anyone have experience about this. Best wishes

08 September 2015 2,270 4 View

Bacterial Protein İsolation?

Can you give some advice? i want extract total protein from klebsiella with/without kit?

04 May 2015 5,581 1 View

How can I measure gene expression treated with antibiotic?

Question is how can I measure gene expression levels of resistance genes treated with antibiotics?

04 May 2015 2,801 6 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Fadaka Adewale, Oluwaseun

Since the length of the primer can help in size of the DNA ladder, Agarose gel electrophoresis with Standard (known kB) Ladder will help you.

DNA MARKER AND LADDER from NEB

Yakubu Pwajok Mwanja

To find amplicon size you have to run a Reverse Transcriptase-quantitative Polymerase Chain Reaction (RT-qPCR).