Which method is the best to study adaptive antibitoic resistance in gram negative bacteria?

09 September 2016 0 7K Report

Which method is the best to study adaptive antibitoic resistance in gram negative bacteria? How many days need to exposure bacteria with sub mic level of antibiotic?

Badges
Science topic

More İbrahim Türkel's questions See All

Biofilm susceptibility testing?

Hi everyone i want determine antibiotic susceptibility of biofilm-state bacteria to compare with planktonic-state bacteria. i searched some papers and met some description like MBEC, "MBEC" is the...

01 February 2017 2,864 5 View

Can i use 2-ΔΔCt and 2-ΔCt formulas together for real time data analyze?

Since getting data from my real time pcr experiments, i'm struggling to analyze these datas. i have 100 bacteria strain isolated from different patients and i want to detect gene expression...

03 April 2016 3,046 11 View

Which method should i use for real time pcr?

Hi all i want to analyze real time pcr results but i have no experience at all. My question is i have Cq value of my gene in resitant strain and suspectibile strain. Also i normalized them with...

03 April 2016 6,907 16 View

Can intermediate (I) value of antimicrobial susceptibility refer as a resistant?

As you know, antibiogram resulted as susceptible (S), intermediate (I) or resistant(R). i also want to detect gene expression between resistant and susceptibel strains. Can i think intermediates...

03 April 2016 4,931 10 View

What must plant contains to have an antibacterial porperty?

Dear Reserachers i want to ask you about plant which have antibacterial. As you know, plants is going to take important point fighting against higly resistance bacteria, fungus etc. Also inhibits...

09 October 2015 8,115 6 View

Any tips for absolute quantification at real time pcr?

Hi all i am new at designing new method for real time pcr and i want to detect some gene expression at klebsiella pneumoniae. i am using 5 ng cDNA template for reaction. My question is which...

09 October 2015 4,125 7 View

Does anyone have experience about plasmid interaction between essential oils and plasmid DNA?

Hi all we want to test plants essential oil vis destilastion method and want to try interactions with plasmid. Anyone have experience about this. Best wishes

08 September 2015 2,270 4 View

Detection amplicon size via primer?

Hi all is there any tool to find amplicon size with forward and reverse primer known only?

07 August 2015 9,499 2 View

Bacterial Protein İsolation?

Can you give some advice? i want extract total protein from klebsiella with/without kit?

04 May 2015 5,581 1 View

How can I measure gene expression treated with antibiotic?

Question is how can I measure gene expression levels of resistance genes treated with antibiotics?

04 May 2015 2,801 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View