Biofilm susceptibility testing?

Hi everyone i want determine antibiotic susceptibility of biofilm-state bacteria to compare with planktonic-state bacteria. i searched some papers and met some description like MBEC, "MBEC" is the...

01 February 2017 2,736 5 View

Which method is the best to study adaptive antibitoic resistance in gram negative bacteria?

Which method is the best to study adaptive antibitoic resistance in gram negative bacteria? How many days need to exposure bacteria with sub mic level of antibiotic?

08 September 2016 7,342 0 View

No title

Since getting data from my real time pcr experiments, i'm struggling to analyze these datas. i have 100 bacteria strain isolated from different patients and i want to detect gene expression...

03 April 2016 2,875 11 View

Can intermediate (I) value of antimicrobial susceptibility refer as a resistant?

03 April 2016 4,797 10 View

Which method should i use for real time pcr?

Hi all i want to analyze real time pcr results but i have no experience at all. My question is i have Cq value of my gene in resitant strain and suspectibile strain. Also i normalized them with...

03 April 2016 6,776 16 View

What must plant contains to have an antibacterial porperty?

Dear Reserachers i want to ask you about plant which have antibacterial. As you know, plants is going to take important point fighting against higly resistance bacteria, fungus etc. Also inhibits...

09 October 2015 7,995 6 View

Any tips for absolute quantification at real time pcr?

Hi all i am new at designing new method for real time pcr and i want to detect some gene expression at klebsiella pneumoniae. i am using 5 ng cDNA template for reaction. My question is which...

09 October 2015 3,955 7 View

Detection amplicon size via primer?

07 August 2015 9,365 2 View

No title

Can you give some advice? i want extract total protein from klebsiella with/without kit?

04 May 2015 5,452 1 View

How can I measure gene expression treated with antibiotic?

Question is how can I measure gene expression levels of resistance genes treated with antibiotics?

04 May 2015 2,675 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View