Hi everyone
i want determine antibiotic susceptibility of biofilm-state bacteria to compare with planktonic-state bacteria. i searched some papers and met some description like MBEC, "MBEC" is the same meaning with "MİC of biofilm cells". and is there any method for Escherichia coli.
Best wishes
Dear ibrahim, my suggestion to check first susceptibility of your strain or strains against antibiotics on broth dilution assay to determine MIC than grow bacteria in 96-well plates and allow them to form biofim (if they are biofilm formers) then add antibiotics and compare with 0 concentration. Biofilm formation is a thick layer and antibiotics can not eradicate easily from the surfaces. Many factors can affect biofilm formation of bacteria (pH, sugar content of media, ionic strength, etc). That is my suggestion.
Best regards.
Hello Ibrahin,
You can always try viability assays and assess prior and post antimicrobial exposure. For example resazurin assesses cell viability.
Good luck!
MBEC is biofilm eradication concentration in which impact of compounds is assessed on pregrown biofilm. MBIC is similar to MIC.
Generally crystal voilet assay is done for quantifying, resazurin assay is also used to assess the viablility.
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