İbrahim Türkel

12 Questions 26 Answers 0 Followers

Questions related from İbrahim Türkel

Biofilm susceptibility testing?

Hi everyone i want determine antibiotic susceptibility of biofilm-state bacteria to compare with planktonic-state bacteria. i searched some papers and met some description like MBEC, "MBEC" is the...

02 February 2017 2,815 5 View

Which method is the best to study adaptive antibitoic resistance in gram negative bacteria?

Which method is the best to study adaptive antibitoic resistance in gram negative bacteria? How many days need to exposure bacteria with sub mic level of antibiotic?

09 September 2016 7,431 0 View

Which method should i use for real time pcr?

Hi all i want to analyze real time pcr results but i have no experience at all. My question is i have Cq value of my gene in resitant strain and suspectibile strain. Also i normalized them with...

04 April 2016 6,858 16 View

Can i use 2-ΔΔCt and 2-ΔCt formulas together for real time data analyze?

Since getting data from my real time pcr experiments, i'm struggling to analyze these datas. i have 100 bacteria strain isolated from different patients and i want to detect gene expression...

04 April 2016 2,994 11 View

Can intermediate (I) value of antimicrobial susceptibility refer as a resistant?

As you know, antibiogram resulted as susceptible (S), intermediate (I) or resistant(R). i also want to detect gene expression between resistant and susceptibel strains. Can i think intermediates...

04 April 2016 4,881 10 View

Any tips for absolute quantification at real time pcr?

Hi all i am new at designing new method for real time pcr and i want to detect some gene expression at klebsiella pneumoniae. i am using 5 ng cDNA template for reaction. My question is which...

10 October 2015 4,072 7 View

What must plant contains to have an antibacterial porperty?

Dear Reserachers i want to ask you about plant which have antibacterial. As you know, plants is going to take important point fighting against higly resistance bacteria, fungus etc. Also inhibits...

10 October 2015 8,072 6 View

Does anyone have experience about plasmid interaction between essential oils and plasmid DNA?

Hi all we want to test plants essential oil vis destilastion method and want to try interactions with plasmid. Anyone have experience about this. Best wishes

09 September 2015 2,224 4 View

Detection amplicon size via primer?

Hi all is there any tool to find amplicon size with forward and reverse primer known only?

08 August 2015 9,445 2 View

Bacterial Protein İsolation?

Can you give some advice? i want extract total protein from klebsiella with/without kit?

05 May 2015 5,537 1 View

How can I measure gene expression treated with antibiotic?

Question is how can I measure gene expression levels of resistance genes treated with antibiotics?

05 May 2015 2,759 6 View

How can the exponential phase of klebsiella pneumoniae bacteria be determined?

Hi all i want to ask you that how can i determine exponential phase of klebsiella pneumoniae? i will try to isolate rna from bacteria and i dont know how many hours the exponential phase takes and...

03 March 2015 2,486 8 View