I want to design a peptide molecule for competitive inhibition of a structure or sequence based protein protein interaction intervention. Please suggest me web server, or literature to start over it.
Thanks in advance.
Hello Everyone, I am culturing few different kinds of adherent cells. However, I sometimes experience cells dying in the flask after passaging them in one flask whereas same cells suspension...
07 February 2024 4,354 0 View
I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...
18 January 2024 958 1 View
20 December 2023 4,363 0 View
What should be the final conc of PI used?
11 October 2023 587 2 View
Hello everyone, I have prepared SnO2 nano powders using chemical co-precipitation method. Eventually, I want a colloidal solution of SnO2. Please guide me how I can convert the nanopowder to...
23 August 2023 7,622 6 View
I want to know about the mechanism through which biological nitrogen fixation occurs in photosynthetic green microalgae apart from previously reported bacterial associations. Like in axenic lab...
11 August 2023 3,256 2 View
In our lab we have cell culture wares for adherent cell lines which are coated. Can I use the same flask for culturing suspension cell line (Jurkat)?
25 June 2023 4,412 2 View
Dear Researchers, I am trying to sputter Cu. I am facing problem to decide the values of different parameters like sputtering time, power, and pressure, such that I will obtain a desired...
07 June 2023 7,092 4 View
Can I calculate the optical energy band gap from the absorbance of the UV-Vis spectrophotometer, or It can only be calculated from the reflectance? Thank you
27 March 2023 9,503 1 View
we have synthesized anticancer drugs which are toxic to cancer cells. but it is desirable that such drugs are non toxic to normal cells. pls provide me a list of normal cell lines where such...
13 March 2023 2,516 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View