Hi all,

I am running serum samples through reverse-phase protein microarrays (RPPM) and I had a large number of samples so I needed to split them between two runs. We like to have samples overlap between both runs for data-analysis purposes therefore I prepped the overlapping samples in the same tubes (.2% SDS, 8.75% glycerol, 1x tris-buffered saline, 1x phosphate-buffered saline, 1x T-per lysis buffer, 1x HALT protease and phosphatase inhibitor, and 1x TCEP bond breaker) and denatured them at 70C for 10 min.

I put the first half of the prepped overlap samples through the assay while the second half sat in the fridge overnight. Upon entering lab today, I discovered that the machine responsible for depositing the prepped samples onto the slides failed to initialize.

Since the machine run time is over 9 hours, I would be unable to process the second half of the samples until next Tuesday (this weekend is a holiday weekend that I am taking a trip with my family).

My question is, will the second half of the prepped/denatured serum samples be stable enough to run after storage at 4C over the weekend?

I would freeze them but I do not want to introduce a freeze/thaw cycle to the second half that was not present in the first half.

Any help is much appreciated!