I do not like how my Keyence BZ-X710 images have been coming out lately. I’ve been noticing grid-like shadows on the images after stitching them, but not while looking at the live images. The Keyence halide bulb was the original culprit, but the problem is still apparent despite it being replaced. I care about this because I’m looking to quantify protein expression, which feels pointless if the image brightness and contrast looks messed up. Slices are 40 microns and are stained for IBA1, GLT1, and DAPI. They’ve only been imaged 1-2 times.
What do people think could be causing this? How do you recommend I go about fixing this?
Anything help. Thanks in advance!
I think this grid like shadows because of diffrence in borders lightening.
To refuse it you must select border removed region of interest in camera setting Or if not availabe adjust microscope condenser hight and pupil to have uniform light.
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