The semen concentration will then be diluted with fraction B (9% glycerol and 1.5% Equex Paste 13560-0008) at 50 °C to a final concentration of 1 x 106 spermatozoa/mL.
This research question explores the complex dynamics of interactions between the oral microbiome and the host immune system, particularly in the development and treatment response of periodontal...
09 July 2024 7,922 1 View
I am interested in looking at male attachment, including the development of attachment wounds, systemic and cultural factors. I’d like to specifically tie together the potential impact of healing...
10 February 2024 655 2 View
I'm working with a student overseas who wants to bring environmental DNA (water filters preserved with ethanol) to my laboratory in the U.S. for a research project. It is not entirely clear to me...
03 December 2023 5,968 2 View
I do not like how my Keyence BZ-X710 images have been coming out lately. I’ve been noticing grid-like shadows on the images after stitching them, but not while looking at the live images. The...
14 September 2023 7,393 1 View
Dear all, I'm currently looking for a dataset with labelled (unenhanced) CT brain images, with at least the ventricles and the brain matter segmented. I've already looked through the links on...
24 August 2023 5,611 1 View
What additives or lubricants are required for the operation status of the steel ball in the fixed section of the CVJ of the car, or at what friction coefficient can the steel ball not move at a...
05 August 2023 2,932 1 View
Normmally you would go from pFastBac to DH10Bac or paceBac to Multibac. I currently have paceBac and DH10Bac and want to avoid ordering multibac if possible. Thanks!
20 April 2023 6,329 2 View
I am currently working with a data set where I would like to answer the research question using a linear mixed models approach. I would like to introduce 2 categorical covariates into the model,...
11 October 2022 6,866 3 View
Hi All, I have only recently started including F and Cl in my microprobe analyses. I am curious how to calculate a mineral formula including F and Cl when assuming a number of oxygens. For...
08 March 2022 7,634 3 View
Hi, Im working on my graduation paper and i'm trying to Identify plant species using machine learning in Erdas imagine. After a few days of getting used to the program and setting up an spatial...
18 January 2022 3,737 4 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,621 4 View
I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...
30 July 2024 968 0 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,591 3 View
I did some column tests in my lab where I needed to detect the DOC fractions using LC-OCD. For the abiotic columns I used sodium azide to restrict the biofilm formation. I did LC-OCD to detect the...
07 July 2024 468 0 View
Bonjour, j'ai une question si quelqu'un a une réponse. J'ai une colonne Uptisphere Strategy 100 Å Bonding HILIC-HIT avec une taille de particules de 3 µm et une longueur de 100 mm. Je n'arrive pas...
24 June 2024 7,201 1 View
Apart from XRD analysis, I want to know the other techniques used for identifying the oxide phases (even when a particular oxide is having very less volume fraction) present in a thermally grown...
14 June 2024 3,555 5 View
how avoid forming lumps in disolving Laemnili buffer 5x? due to presence of undissolved bromoclesol blue and thick glycerol. Please tell me how to resolved this issue.
12 June 2024 10,034 3 View
I used a two-phase extraction protocol (essentially the Bligh-Dyer or Folch protocol) to fractionate C. elegans into aqueous metabolites, lipids, and protein. These worms have been fed bacteria...
11 June 2024 8,991 1 View
I've pelleted down the overnight ampicillin supplemented cultures of DH5ALPHA for pET22b isolation 5 days ago and stored them on -20°C without adding any buffer or glycerol. Would the cells be...
09 June 2024 6,479 2 View
Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...
09 June 2024 6,650 9 View