Direct PCR lysis buffer is one of the fastest methods to do genotyping. By taking a piece of tissue and placing it in a small volume of Direct PCR lysis buffer you can then take a few microliters and do a PCR directly. I would like to do a similar approach but performing a restriction enzyme digestion instead of a PCR. The issue is that the DNA is in a buffer of unknown composition and I am not sure whether this will affect the restriction enzyme reaction. Have anyone experience with that? Thanks! Miguel
If the buffer composition is similar to the requirements of the restriction enzyme, it might work, this is rather easy to test.
As you are lysing tissue, I'd expect there are proteases like e.g. Proteinase K, which usually require a heating step for inactivation. Otherwise they might chew up your restriction enzyme.
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