Miguel Vizoso

4 Questions 2 Answers 0 Followers

Questions related from Miguel Vizoso

Derived organoids stop growing after first week then losing the cystic morphology and adcquiring a blob-like morphology?

Dear all, I have been growing mouse organoids and they grow well at first. I am using the general media and conditions suggested by Clevers´lab and other labs. Before I provide more in detail...

22 July 2023 6,040 3 View

Levels of estradiol in intact females odderly high?

Dear all, I am measuring the levels of estradiol in plasma in intact B6 females around 18 weeks old, using QuickStep Mouse Estradiol ELISA competitive assay (QSES033). I am getting for all mice...

22 April 2023 3,362 0 View

Dear all, I was wondering if someone has tried to do a restriction enzyme digestion using DNA extracted by Direct PCR lysis buffer?

Direct PCR lysis buffer is one of the fastest methods to do genotyping. By taking a piece of tissue and placing it in a small volume of Direct PCR lysis buffer you can then take a few microliters...

28 April 2022 5,736 1 View

Neutrophil activation during isolation

Dear all, I am trying to isolate PBMCs from human blood. I am doing a gradient centribugation to remove most of the erythrocytes. The leftover erythrocytes I get rid of them using ACK lysis buffer...

01 January 1970 9,763 1 View