I'm planing to use human Fibroblasts (Bj hTERT) for this assay
Dear Karim,
I would recommend ours, you can find the reference in the link below,
please feel free to contact me via Researchgate or directly at: [email protected] for any question
best regards,
Cedric
http://www.ozbiosciences.com/assays-kits/61-senescence-kit.html
Thanks Cédric for the suggestion.
But, why you don't use the home-made solutions? They are easy to make, they are cheap and work nice. Don't behave as a kit generation product.
The attached article could (maybe) of interest ...
Best regards
Robert
I've had good experiences with the one marketed by Cell Signaling Technology (product number 9860) when using it on bovine fibroblasts.
https://www.merckmillipore.com/IT/it/product/Cellular-Senescence-Assay,MM_NF-KAA002?ReferrerURL=https%3A%2F%2Fwww.google.it%2F&bd=1
I also heard that the solutions are easy to make and the assay not as tricky as it would deserve a kit.... but my first tril will only be in 2 weeks
I have a flow flux data and want to use it for calibration. Can you please tell me step by step how to do it?
15 May 2024 8,180 0 View
Hello Everyone ! I have few queries regarding SCM-COSMO RS. Is there anyone working on CO2 interaction with ionic liquids using SCM-COSMO RS? Can we use optimized files from Gaussian to...
15 May 2024 4,786 0 View
Teachers' personal and professional lives seem to be intertwined. In your opinion, how do teachers' personal lives impact their roles as teachers?
09 May 2024 4,632 6 View
Please ask if you need additional information.
05 May 2024 8,580 2 View
Extraction of essential oils from plants.
30 April 2024 7,102 2 View
hi everyone, I am currently working on my thesis, which consists of 6 nations with 23 years. my model is UNEM = ECONOMIC DIVERSIFICATION INDEX + INF + LnPOP + FDI + ICT, since my model face a...
19 March 2024 4,569 0 View
This question is very important because its answer is “ don’t and impossible to contain Fossils. This is true and universally 100% accepted, but a new paper published in High Impact Factor (6.5)...
15 March 2024 7,534 4 View
Chemical equilibria in soils free download
23 February 2024 3,623 0 View
I am working on AA based protic ionic liquids. How can we confirm that a proton from amino acid has been transferred to cation to form an ionic liquid??
19 February 2024 693 2 View
If anybody done this type of work,please answer me.
18 February 2024 290 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks
03 July 2024 9,866 2 View
Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...
23 June 2024 4,902 3 View
Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable
15 June 2024 3,010 2 View
I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...
12 June 2024 7,331 1 View
Which is the best method for the characterization of beta cyclodextrin complex formation with guest molecule in solution form and in solid form ?
11 June 2024 6,719 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View
Hi everyone, I am working with an adhesive fibroblast cell line in roller flasks (Cell Master Bottles). When culturing the cells with FBS that is heated with FBS (around 60 degrees C), the cells...
02 June 2024 7,409 1 View