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Questions related from Vanessa S
I bought an inhibitor (Jedi1; CAS 108124-17-0 for who wants to check the structure) and my colleague put the poder upon arrival in the freezer instead of the fridge. It was there just over night...
12 April 2023 7,150 5 View
Tricky or stupid question : I measure in different cell lines Ca-oscillation upon stimuli over a period of 4-6min. I use FLuo-4-AM dye from Thermo Fisher, btw 3 and 4 microMolar. Incubation in...
26 October 2021 3,501 0 View
Dears, I used 4% PFA to stain in vitro cultured cells for protein staining (Cristal Violet). No I would like to stain lipid droplets in the cell with oil-red solution. I thought I will NEED...
07 February 2020 2,272 1 View
I work with Xenografts, hence human tumor cells of a breast cancer model. I would now like to stimulate the tumor growth with leptin and was wondering whether murine or human leptin would bind...
07 November 2018 8,982 0 View
Hello, I want to analyze the level of activity of some CDK's in tumors (xenograft) that I have snap frozen in liquid nitrogen. I found this protocol A new cancer diagnostic system based on a...
06 June 2018 4,461 0 View
Hello all, I am working with hormone responsive cancer cells (MCF7, ER positive) and wondering whether I need to use stripped serum or not . In literature, some people either strip their...
07 October 2017 10,105 3 View
Hello everybody, I tried to isolated cancer cells from a tumor mass (xenograft). I tried two different protocols where masses were initially digested with Collagenase + Hyaluronidase, in a...
05 August 2017 8,551 3 View
Hello all, I want to re-isolate MCF7 cells from xenograft masses and grow them in vitro (just a wee or two). In different protocols I red that people use always colagenases but sometimes also...
31 July 2017 6,195 2 View
Hello everybody, I want to try the comet assay, which is new for me. in order to see whether my treatments induce directly DNA strand breaks or via ROS formation I'd compare samples run with the...
12 April 2017 3,675 3 View
Hello, I need to start a quite big mouse experiment and have right now F1 mice (parents were bought form a company) as breeding couples. They wont give enough mice though to start the experiment...
18 October 2016 7,851 3 View
Hello, I am interested in aquired resistance upon Tamoifen and Fulvestrant in hormoe responsive breast cancer. I work basically with MCF7 cells, but also T47D and ZR 75-1 , all ER positive. When...
05 September 2016 2,182 0 View
Hello, I am testing the effect of pharmaceuticals against estrogen responsive breast cancer - using MCF7 cells in NOD SCID IL2RGnull mice. I transplant usually an estrogen releasing pellet under...
31 August 2016 5,782 6 View
Does anyone have a protocol to make your own estrogen free Serum ? I heard you can get the charcoal and stripp it your own, but can't find a protocol with refrence... And do you know what else is...
16 May 2016 3,843 1 View
Hello, I tested different drugs for cancer treatment in vitro for now. As I followed so far a previously set up protocol, it just came now into my mind, that the drugs half life might play a more...
09 February 2016 2,790 4 View
I bought 10mg RNAse from Sigma, which comes in a drop of 50% Glycerol and Tris - HCl. I want to add 1mL solution to bring it to a nice working concentration of 10mg/mL. Now we discuss in the Lab...
08 February 2016 5,905 3 View
Hello, I need to use Tamoxifen in vitro. Sigma offers (Z)-4-Hydroxytamoxifen (http://www.sigmaaldrich.com/catalog/product/sigma/h7904?lang=en®ion=IT) which is described as the active...
02 February 2016 334 7 View
If I need to treat cell lines with drugs, that are (or are not) FDA approved, and I find the dosis used in human or mice....(I find it often on the FDA website), how can I calculate or where can...
01 November 2015 3,721 16 View
I heard that you should store DMSO not to long in a plastic falcon, but always take it fresh from the dark glass bottle. Is that true ? and if yes, does anybody have an explanation ? I can't find...
26 June 2015 8,899 9 View
I want to dissolve my Ibrutinib in pure DMSO, it can be stored at -20°C according to the company. They unfortunately do not specifiy if there is a limit to thaw and refreeze the drug without...
24 April 2015 9,890 4 View
Hey, 1) I am performing MTT assays and was wondering how much the cell size can influence the mount of metabolized MTT? I thought that the amount of mitochondrial membrane, (so also the amount of...
29 March 2015 4,538 7 View
Does anybody know what is the highest concentration of a Stock solution of MTT (powder from Sigma ) in Culture Medium ? I know from Sigma that they tested their powder up to 5mg/ mL and that in...
29 March 2015 5,763 5 View
I am currently searching for a new citation program (used EndNote in the past) and came across Docear, which seems to be the only one offering also a mind map tool. But after downloading I had to...
01 January 1970 2,623 1 View
