Vanessa S

12 Questions 22 Answers 0 Followers

Questions related from Vanessa S

What happens to Fluo-4-AM dye during decrease of Ca concentration during long terma acquisition ?

Tricky or stupid question : I measure in different cell lines Ca-oscillation upon stimuli over a period of 4-6min. I use FLuo-4-AM dye from Thermo Fisher, btw 3 and 4 microMolar. Incubation in...

26 October 2021 3,444 0 View

Can 4% PFA in methanol free PBS fix AND permeabilize cells ?

Dears, I used 4% PFA to stain in vitro cultured cells for protein staining (Cristal Violet). No I would like to stain lipid droplets in the cell with oil-red solution. I thought I will NEED...

07 February 2020 2,169 1 View

Does Serum derived from the fetus contain hormones?

Hello all, I am working with hormone responsive cancer cells (MCF7, ER positive) and wondering whether I need to use stripped serum or not . In literature, some people either strip their...

07 October 2017 10,035 3 View

WHEN using WHICH enzyme mix to isolate tumor cells from xenograft masses ?

Hello all, I want to re-isolate MCF7 cells from xenograft masses and grow them in vitro (just a wee or two). In different protocols I red that people use always colagenases but sometimes also...

31 July 2017 6,139 2 View

Who's experience with the Comet assay, knows how to save costs especially for use of enzymes ( hOGG1,FPG or EndoIII)?

Hello everybody, I want to try the comet assay, which is new for me. in order to see whether my treatments induce directly DNA strand breaks or via ROS formation I'd compare samples run with the...

12 April 2017 3,617 3 View

Can I mix F2 and F3 generations in 1 big mouse Experiment?

Hello, I need to start a quite big mouse experiment and have right now F1 mice (parents were bought form a company) as breeding couples. They wont give enough mice though to start the experiment...

18 October 2016 7,802 3 View

What is the best strain to do MCF7 Xenograft using estrogen pellet ?

Hello, I am testing the effect of pharmaceuticals against estrogen responsive breast cancer - using MCF7 cells in NOD SCID IL2RGnull mice. I transplant usually an estrogen releasing pellet under...

31 August 2016 5,660 6 View

How can I make my own estrogen free Serum ?

Does anyone have a protocol to make your own estrogen free Serum ? I heard you can get the charcoal and stripp it your own, but can't find a protocol with refrence... And do you know what else is...

16 May 2016 3,779 1 View

Can RNAse be diluted in PBS and (re) frozen ?

I bought 10mg RNAse from Sigma, which comes in a drop of 50% Glycerol and Tris - HCl. I want to add 1mL solution to bring it to a nice working concentration of 10mg/mL. Now we discuss in the Lab...

08 February 2016 5,856 3 View

How do I calculate from in vivo or patient drug concentration the corresponding in vitro drug concentration?

If I need to treat cell lines with drugs, that are (or are not) FDA approved, and I find the dosis used in human or mice....(I find it often on the FDA website), how can I calculate or where can...

01 November 2015 3,649 16 View

Is DMSO less stable when stored in plastic(Falcon) instead of glass?

I heard that you should store DMSO not to long in a plastic falcon, but always take it fresh from the dark glass bottle. Is that true ? and if yes, does anybody have an explanation ? I can't find...

26 June 2015 8,843 9 View

Citation programs INCLUDING mind map tool

I am currently searching for a new citation program (used EndNote in the past) and came across Docear, which seems to be the only one offering also a mind map tool. But after downloading I had to...

01 January 1970 2,559 1 View