Tom20 antibodies for mice?

Hello, Does anyone know tom20 antibodies that work well for mice? I tried Tom20 Antibody (F-10 and 29) and both did not work very well. Thanks!

31 December 2019 1,162 1 View

FLT3L bone marrow dendritic cell - what is the best marker?

Hello, What would be the best marker for FLT3L-induced bone marrow dendritic cell for flow cytometry?

08 September 2018 6,653 0 View

Cell counting using flow cytometry?

All- Is there a way to count lymphocytes from purified spleen using flow cytometry that will give comparable cell # from hemocytometer?

11 December 2017 9,789 4 View

Cell viability using flow cytometry vs MTT?

All- I was wondering if you can use cell viability test using live/dead for flow cytometry in lieu of MTT assay? Is this acceptable?

10 November 2017 8,863 3 View

What is the best way to measure the activity of NOX2 using flow cytometry or biochemical method?

All - I saw different methods of measuring NOX2 activity using luciferin-based or NADPH reduction and etc.. What is really the best way of accessing this activity? Thanks!

10 November 2017 8,306 1 View

4 color imaging on confocal sp8?

Hello all- Is there a way to image 4 colors on Leica SP8? We have UV (405 nm), and 45, 476, 488, 496, 514, 561, 633 nm. I want to use.. AF 488 AF 594 AF 647 I really want to avoid lower than 488...

05 June 2017 2,822 0 View

LC3 I/II and p62 weird ratio?

Hello all, I treated with a type of adjuvant I am interested in with DC 2.4 cell lines (DC-like murine cells) for 3, 6, 12, and 24 hrs interval. I then lysed the whole cell extract and did WB...

03 April 2017 9,122 2 View

ImageStream - Co-localization?

11 December 2016 8,546 0 View

FC block for intercellular staining for flow cytometry?

Dear all, I have a lot of non-specific bindings for one of the staining for intercellular staining in cells derived from mice. My 1' antibody is from rabbit. If I am doing just one color for ICCS,...

10 November 2016 6,752 4 View

96 wel culture-treated flat bottom plate - how to retrieve the cells?

09 October 2016 3,793 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View