Is there a way to batch-convert mass spectrometry cdf files to mzML files?

I have a set of roughly one hundred cdf files from a mass spectrometry experiment. I would like to analyse them using MetaboAnalystR, which requires mzML files as input. I know I can convert each...

28 March 2020 1,794 6 View

What is the best methodology for working with temporal data holes and deep neural networking?

Any data science and/or remote sensing experts have any insight pertaining to multivariate regression and deep neural networking with large feature set inputs and variable temporal data holes...

03 February 2020 5,246 2 View

Why are surrogates only added at the time of extraction, not at the time of sample collection and processing?

I am working on a study on pharmaceutical fate and uptake in plants. We are wondering about analyte degradation during the initial transport, processing, and storage of the soil and plant samples,...

30 October 2019 3,540 1 View

Is there a method or reference that shows how (or what is) the oxygen transmission rate (OTR) of water?

I have found multiple references on solubility however I can find nothing on transmissibility. The later is usually reserved for polymer films but in theory it should be determinable for water as...

05 August 2019 4,776 3 View

How is depth information encoded in the frequency of spectral interferograms obtained by Spectral domain optical coherence tomography?

I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length. From what I have gathered so far, it seems that the...

10 June 2019 6,783 2 View

How can I improve laser penetration on a confocal microscope in aortic tissue?

I am currently doing an experiment to image thick (~100 micron) cut pieces of aorta and am having a few problems. To imagine the shape of my tissue, I am cutting rings or bands of aorta and...

01 August 2018 920 3 View

Can BLAST perfrom a search including IUPAC ambiguity codes?

23 June 2017 8,927 3 View

What is the expected number of connected components in an Erdos-Renyi graph?

Let G(N,p) be an Erdos-Renyi graph, where N is the number of vertices, and p is the probability that two distinct vertices form an edge. If I plot 1-b0/N over log(p), then I obtain a curve which...

23 November 2016 6,443 3 View

How to conduct measured path analysis using Lisrel?

I am trying to understand how to use Lisrel to produce the best model for my survey data. I have read through the guides provided by the website but still do not seem to understand the...

19 February 2015 3,277 5 View

How should one handle 0 or low cell sizes in ordinal/multinomial logistic regression?

Hi all, I am running into a snag creating a path analysis model using ordinal and multinomial logistic regression. I am using scaled scores as predictors of either an ordinal (OnlineSatisfaction,...

12 February 2015 1,994 8 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View