I have to check that whether my purified protein interacts with DNA for which i need to perform an EMSA. I am unable to design this experiment properly so need some tips and suggestions. Thankyou in advance.
I want to calculate the Mean absolute percentage error (MAPE) for my copula model. I am stuck at the forecasting step. I am not specifying the copula here for different data pairs. 1. I have two...
14 July 2024 7,604 1 View
Hi, I have parameters of time-varying normal copula. Now I want to forecast using these parameters. For that I will need to generate data using these copulas parameters as shown in the...
20 May 2024 488 0 View
i am refolding protein by gradient dilaysis in which i gradually decrease concentration of urea by gradient dialysis. however im not getting surety wheather denaturant has been completely removed...
24 April 2024 5,399 1 View
Hello, I have read that for standard copula modeling, you can get empirical cdf of data and use it for copulas. But for time series data, we must first fit ARIMA/GARCH, get standardized...
11 February 2024 2,463 1 View
Hello, All papers I have read about time-varying copulas just produce tables, but none explains exact steps for empirical data. How do I calculate the time varying parameters for the copula? I...
18 December 2023 5,453 1 View
I am doing cloning of a big bacterial insert (3705bp) into a vectors of varying sizes ranging from 3017bp to 3469bp for my bacterial two hybrid experiment. Among other problems with my cloning I...
15 November 2023 7,101 4 View
I have 6 different powder samples of Mechanically Alloyed magnetic High Entropy Alloy Powders as FeCoNiAlMn1-xCrx (x=0,0.2,0.4,0.6,0.8,1). I need to measure there microwave absorption properties...
24 July 2023 3,412 0 View
want to visualize SwissDoc protein-ligand docking results in ChimeraX.
22 July 2023 7,186 0 View
I have latest Chimera X version and I want to make an animation without using command line method. first I do not see animation tab under utilities tab. Why? second I am unable to save or encode...
29 June 2023 7,034 0 View
When we do GARCH, we make time series stationary. Stationary means constant mean and variance. Then how are we modelling variance with GARCH when its stationary? What am I missing here. Also, can...
21 June 2023 9,165 5 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View