Hi! I have a question about mycoplasma test....we use qPCR kit to check the presence of mycoplasma in cells, and hystorically we used to eliminate antibiotic from cell culture medium at least 1 week before the mycoplasma test. I'm a little doubtful about the usefulness of this step. Do you have experience in mycoplasma test with antibiotics (I mean amp, Kana and so on)?
It's the deprivation of the antibiotic necessary to have affordable results?
Thanks in advance!