CTAB substitute in DNA isolation?

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¿Quienes investigan en Argentina o Iberoamérica sobre enseñanza de las Ciencias Naturales desde una perspectiva interdisciplinaria?

Necesito publicaciones o los nombres de quienes hayan o estén trabajando en ensenanza interdisciplinaria de las Ciencias Naturales. Particularmente me interesa sobre formación docente y currículum...

21 May 2023 5,452 4 View

Are there investigations on the impact of social networks in chilhood?

What kind of studies had been published about the use of social networks in kids?

29 March 2023 6,453 0 View

How can I calculate the TARC value and group delay in CST for a MIMO configuration?

Post proccessing in CST

14 June 2022 4,821 2 View

How can I increase a polymer’s melt viscosity without increasing Mw or introducing cross linking agents?

I am trying to raise the melt viscosity of a PHA polymer however as it is microbially produced the MW is already very high and difficult to control, and the addition of crosslinking agents will...

26 April 2022 5,160 6 View

What is the minimum impactful target coral restoration area (km2) within the Coral Triagle?

Assume that the current coral cover is between 20% and 30%, down from 40% over the past 10 years and that the restoration project can be funded for at least 10 years.

22 July 2021 3,320 3 View

How can I visualize an objective function in a multi-variable design space?

Hello everyone! I hope you can help me or give advice on the following. In optimization, it is common practice to draw the objective function over the design space to assess the optimal solution....

23 November 2020 3,021 0 View

Do you know of studies dealing with the contamination or not of vegetables cultivated using compost from the residues of dry toilets?

In many countries, eating vegetables produced with compost from the toilet is prohibited. Is there really a big problem?

12 June 2020 4,481 1 View

What are the disadvantages of reusing composts from the composting of residues from dry toilets with sawdust in agriculture?

In some European countries, in Africa, in Latin America and even in the Caribbean, individual sanitation by dry litter toilets is practiced by some communities. The residues from these toilets are...

12 June 2020 2,887 1 View

Other than bagasse, what sawdust could help reduce the time it takes to sanitize residues from dry litter toilets by composting?

During a composting experiment, it was found that the best results are therefore obtained with a litter made of 100% bagasse, which may be due to the presence of residual sugar, the biodegradation...

14 May 2020 506 3 View

Does it exist low tech treatments of WEEE, waste of electric and electronic equipment, adapted to low income countries, to recover the pure metals?

Often people dismantle WEEs just in pieces and send them in Northern countries. They so loose most of the add value of this activity.

08 May 2020 8,750 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View