How can we perform the skin sampling from psoriasis-like mice model for immunohistochemistry?

Hello everyone ! I am willing to sample the mouse skin for immunohistochemistry analysis from the mouse having psoriasis-like symptoms in dorsal skin layer. Could you please help me how should I...

07 August 2017 4,066 1 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

08 September 2016 6,826 1 View

How can we determine the phenotype of activated microglia through its morphology?

Hello ! I am working on microglia phenotype study following focal cerebral ischemic challenge in mice. Through the analysis of different biochemical markers (by qPCR, and western blot) we can...

08 September 2016 2,879 9 View

Is it okay to use water while diluting cDNA or primers?

Previously we used to use RNAse free water (DEPC water) for all genetic experiments that is RNA extraction, cDNA synthesis, semi-PCR or RT-PCR. Recently we use DEPC water only for RNA work. For...

03 April 2016 3,781 5 View

Could you please let me know the size and length of monofilament for tMCAo induction in C57/BL6 male mice of 8 weeks?

I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I...

02 March 2016 1,208 7 View

Does all experimental interventions are neuroprotective following stroke?

Could you please provide me some article which showed improvement of neurological deficit without change in infarct volume or vice versa after drug treatment flowing stroke induction in rodents.

02 March 2016 7,036 3 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

01 February 2016 7,414 2 View

How to extract proteins from formaldehyde fixed frozen tissue section (any kits) ?

I am planning to extract the proteins for western blot purpose from the fixed brain tissue sections. Brains tissue were fixed in 4% para formaldehyde and frozen at -80 degree Celsius. Do you know...

10 November 2015 2,837 2 View

What would be the best marker for microglia in cerebral ischemia ?

We are using iba1 as the marker for the activation and proliferation study of microglia following focal cerebral ischemia. However iba1 stains both macrophages and microglia thus creating...

09 October 2015 2,506 8 View

How do I dilute DAPI solution (from stock) for immuno-fluorescence assay?

I have DAPI stock solution in DMSO (5 mg/ml) and need it for the use in immuno-fluorescence (IF) assay (mouse brain section). Generally we use 1:10,000 ration of DAPI with PBS in IF assay. At this...

07 August 2015 6,725 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View