03 March 2016 3 7K Report

Could you please provide me some article which showed improvement of neurological deficit without change in infarct volume or vice versa after drug treatment flowing stroke induction in rodents.

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Could you please suggest me for protein quantification using nanodrop 2000c?

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How can we determine the phenotype of activated microglia through its morphology?

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Could you please suggest me the mounting medium/solution for cells ?

I am using VECTA SHELD mounting solution to mount my cells growing in coverslips. I wish to fix my cells using fixative solution and for that I have to use another mounting solution. And moreover,...

03 April 2016 9,733 2 View

Could you please let me know the size and length of monofilament for tMCAo induction in C57/BL6 male mice of 8 weeks?

I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I...

02 March 2016 1,347 7 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

In many cases, neurological score and infarct volumes of MCAo/reperfusion (M/R) induced brain damage correlates. I mean if infarct volume is improved so does the neurological functions. However,...

01 February 2016 7,550 2 View

How to extract proteins from formaldehyde fixed frozen tissue section (any kits) ?

I am planning to extract the proteins for western blot purpose from the fixed brain tissue sections. Brains tissue were fixed in 4% para formaldehyde and frozen at -80 degree Celsius. Do you know...

10 November 2015 3,106 2 View

What would be the best marker for microglia in cerebral ischemia ?

We are using iba1 as the marker for the activation and proliferation study of microglia following focal cerebral ischemia. However iba1 stains both macrophages and microglia thus creating...

09 October 2015 2,635 8 View

How do I dilute DAPI solution (from stock) for immuno-fluorescence assay?

I have DAPI stock solution in DMSO (5 mg/ml) and need it for the use in immuno-fluorescence (IF) assay (mouse brain section). Generally we use 1:10,000 ration of DAPI with PBS in IF assay. At this...

07 August 2015 6,872 5 View

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