How can we perform the skin sampling from psoriasis-like mice model for immunohistochemistry?

Hello everyone ! I am willing to sample the mouse skin for immunohistochemistry analysis from the mouse having psoriasis-like symptoms in dorsal skin layer. Could you please help me how should I...

07 August 2017 4,066 1 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

08 September 2016 6,826 1 View

How can we determine the phenotype of activated microglia through its morphology?

Hello ! I am working on microglia phenotype study following focal cerebral ischemic challenge in mice. Through the analysis of different biochemical markers (by qPCR, and western blot) we can...

08 September 2016 2,879 9 View

Is it okay to use water while diluting cDNA or primers?

Previously we used to use RNAse free water (DEPC water) for all genetic experiments that is RNA extraction, cDNA synthesis, semi-PCR or RT-PCR. Recently we use DEPC water only for RNA work. For...

03 April 2016 3,781 5 View

Could you please suggest me the mounting medium/solution for cells ?

I am using VECTA SHELD mounting solution to mount my cells growing in coverslips. I wish to fix my cells using fixative solution and for that I have to use another mounting solution. And moreover,...

03 April 2016 9,588 2 View

Could you please let me know the size and length of monofilament for tMCAo induction in C57/BL6 male mice of 8 weeks?

I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I...

02 March 2016 1,208 7 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

01 February 2016 7,414 2 View

How to extract proteins from formaldehyde fixed frozen tissue section (any kits) ?

I am planning to extract the proteins for western blot purpose from the fixed brain tissue sections. Brains tissue were fixed in 4% para formaldehyde and frozen at -80 degree Celsius. Do you know...

10 November 2015 2,837 2 View

What would be the best marker for microglia in cerebral ischemia ?

We are using iba1 as the marker for the activation and proliferation study of microglia following focal cerebral ischemia. However iba1 stains both macrophages and microglia thus creating...

09 October 2015 2,506 8 View

How do I dilute DAPI solution (from stock) for immuno-fluorescence assay?

I have DAPI stock solution in DMSO (5 mg/ml) and need it for the use in immuno-fluorescence (IF) assay (mouse brain section). Generally we use 1:10,000 ration of DAPI with PBS in IF assay. At this...

07 August 2015 6,725 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View