29 Questions 53 Answers 0 Followers
Questions related from Bhakta Prasad Gaire
Hello everyone ! I am willing to sample the mouse skin for immunohistochemistry analysis from the mouse having psoriasis-like symptoms in dorsal skin layer. Could you please help me how should I...
08 August 2017 4,168 1 View
Hello ! I am working on microglia phenotype study following focal cerebral ischemic challenge in mice. Through the analysis of different biochemical markers (by qPCR, and western blot) we can...
09 September 2016 2,976 9 View
I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...
09 September 2016 6,925 1 View
Previously we used to use RNAse free water (DEPC water) for all genetic experiments that is RNA extraction, cDNA synthesis, semi-PCR or RT-PCR. Recently we use DEPC water only for RNA work. For...
04 April 2016 3,995 5 View
I am using VECTA SHELD mounting solution to mount my cells growing in coverslips. I wish to fix my cells using fixative solution and for that I have to use another mounting solution. And moreover,...
04 April 2016 9,683 2 View
I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I...
03 March 2016 1,302 7 View
Could you please provide me some article which showed improvement of neurological deficit without change in infarct volume or vice versa after drug treatment flowing stroke induction in rodents.
03 March 2016 7,116 3 View
In many cases, neurological score and infarct volumes of MCAo/reperfusion (M/R) induced brain damage correlates. I mean if infarct volume is improved so does the neurological functions. However,...
02 February 2016 7,507 2 View
I am planning to extract the proteins for western blot purpose from the fixed brain tissue sections. Brains tissue were fixed in 4% para formaldehyde and frozen at -80 degree Celsius. Do you know...
11 November 2015 3,001 2 View
We are using iba1 as the marker for the activation and proliferation study of microglia following focal cerebral ischemia. However iba1 stains both macrophages and microglia thus creating...
10 October 2015 2,584 8 View
I have DAPI stock solution in DMSO (5 mg/ml) and need it for the use in immuno-fluorescence (IF) assay (mouse brain section). Generally we use 1:10,000 ration of DAPI with PBS in IF assay. At this...
08 August 2015 6,824 5 View
2 independent groups are generally tested by t-test for the significant difference analysis. Can we do that by the use of ANOVA? lets say group A has 5 different values, 2,3,4,5,6 and group B has...
04 April 2015 5,184 1 View
I am doing research on stroke animal models and generally use middle cerebral artery occlusion induced focal cerebral model. As you know the infarction degree is quantified by TTC staining and the...
02 February 2015 7,882 6 View
I have to treat the BV2 cells repeatedly for 5 days (daily treatment) with an immuno-modulator. However, the problem is that BV2 morphology changed after 72 hr and they start to die after 4 days....
01 January 2015 265 5 View
While doing the stroke animal research, generally we performed the sham surgery, MCAO (stroke surgery) and stroke + drug treated surgery. In many papers the processes is same. However some paper...
11 November 2014 1,746 2 View
I have to prepare the lecture for the undergraduate students regarding the middle cerebral artery occlusion (MCAo) in mice. I am looking for some videos so that I can explain it clearly to the...
09 September 2014 2,467 1 View
I am doing the research on animal stroke models. Generally we use the MCAo induced focal cerebral ischemic models in mice. As it is obvious that mice undergone with right MCA occlusion circles...
09 September 2014 3,231 2 View
I usually perform the intracerebroventricular injection in mice ventricle with coordinates of -0.3mm anteroposterior, + or - 1 mm mediolateral and -3 mm dorsoventral. Similarly could you please...
09 September 2014 2,023 2 View
This seems a bit nonsense question, however I am confused with this. I am doing the middle cerebral artery occlusion experiment using mice using intramural suture (filament) method. As we know the...
09 September 2014 9,100 13 View
As it is obvious that the level of TNF-alpha is increased following cerebral ischemia. I am going to perform the immunostaining for TNF-alpha positive cells in mice brain sections which is...
08 August 2014 8,449 7 View
I am working with stroke animal models and have to perform the immunohistochemistry time and again. To label the astrocyte I generally used the GFAP marker with 1:500 dilution of the antibody. The...
06 June 2014 4,578 2 View
I am facing problems while counting Iba 1 positive cells in mouse brain section. I cut the brain slices in 20 micron thickness and performed immunohistochemistry. And now I have to perform the...
06 June 2014 9,141 9 View
Please suggest the appropriate solvent.
05 May 2014 2,613 2 View
Iba 1 is used for the immunohistochemical analysis of activated microglial cells. I am doing stroke animal research and frequently encounter the staining with the Iba 1 antibody. My problem is Iba...
05 May 2014 8,614 3 View
I have to perform the intrathecal injection for the mice of about 8 weeks. I am planning to use 31 gauze insulin syringe since we have larger Hamilton syringes only. Do you have any specific...
12 December 2013 5,844 10 View
Gallic acid, ellagic acid and chebulagic acid are the major polyphenols from the fruit of Terminalia chebula and it is reported that some polyphenols like baicalein have the neuroprotective effect...
04 April 2013 7,597 0 View
Do you have any articles regarding the protective effect of Terminalia chebula on the glutamate induced toxicity to the neurons? Both in vitro and in vivo paper will be appreciated. Either the...
04 April 2013 2,373 2 View
I am planning to prepare female MCAo stroke model without estrogen. The removing the ovary of the female SD rat is the popular and most widely used method for that. I have undergone through the...
03 March 2013 4,269 1 View
In my research of the antioxidant activity of T. chebula, extract at a higher concentration showed less antioxidant activity than a lower concentration (0.1- 1 micro gram/ml). In a literature...
01 January 2013 6,691 6 View
