Is there any glycoshpingolipid detection technique based on HPLC or UV?

I want to detect glycosphingolipid by UV detector in HPLC. Please, anyone let me know.

10 November 2018 9,464 2 View

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

08 September 2018 8,079 6 View

How can I measure Glucosylceramide or lactosylceramide by HPLC or spectrometer?

I want to measure glucosylceramide or lactosylceramide quantity by spectrometer or HPLC.

03 April 2018 2,002 4 View

How can I get a gene ontology software for free?

I need to download gene ontology software for free. Thanks

02 March 2018 3,102 0 View

Why double band appeared on TLC plate?

I run standard mixture on TLC plate. All standards showed with double band.

01 February 2018 3,141 4 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

01 February 2018 2,643 3 View

How can I download protein structure having mutation hotspot mark?

I want to download p53 protein structure with hotspot mutation mark from protein data bank. But I could not. Would you suggest me how to use PDB or other website to see protein structure? Thanks

31 December 2017 3,639 1 View

How much sample (in amount) should be loaded on TLC plate?

I am loading 10 microliter sample slution on TLC plate. I want to know the amount of sample should be loaded on plated for detection. Thanks.

31 December 2017 2,895 7 View

How I can separate glycosphingolipids from total cell lipid?

I am trying to extract glycosphingolipid from total cellular lipids. Later I will detect shingolipid by HPLC. Thanks

07 August 2017 6,108 4 View

Could you tell me cancer resistance cells which express Glucosylceramide synthase.

Glucosylceramide synthase (GCS) performs ceramide glycosylation which is important for resistance to chemotherapy. Which cancer resistance cells express more GCS. Thanks

07 August 2017 9,076 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View