I am working with TP53 cell line which is genetically engineered cell line by CRISPR-Cas9 system. Supplier recommended to use Sodium Bi-carbonate in culture medium.
dear karrtik. ye kya pucha he yaar aapne. itna basic sawal he ye. apke question ki wajah se hindi me likna pad raha h.
obviously NaHCO3 is used for buffering of pH in media.
Thanks Arun. My question is why buffering agents is recommended for genetically engineered cells, why not other cancer cell line like SW48?
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