Is there any glycoshpingolipid detection technique based on HPLC or UV?

I want to detect glycosphingolipid by UV detector in HPLC. Please, anyone let me know.

10 November 2018 9,464 2 View

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

08 September 2018 8,079 6 View

How can I measure Glucosylceramide or lactosylceramide by HPLC or spectrometer?

I want to measure glucosylceramide or lactosylceramide quantity by spectrometer or HPLC.

03 April 2018 2,002 4 View

How can I get a gene ontology software for free?

I need to download gene ontology software for free. Thanks

02 March 2018 3,102 0 View

Why double band appeared on TLC plate?

I run standard mixture on TLC plate. All standards showed with double band.

01 February 2018 3,141 4 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

01 February 2018 2,643 3 View

How can I download protein structure having mutation hotspot mark?

I want to download p53 protein structure with hotspot mutation mark from protein data bank. But I could not. Would you suggest me how to use PDB or other website to see protein structure? Thanks

31 December 2017 3,639 1 View

How much sample (in amount) should be loaded on TLC plate?

I am loading 10 microliter sample slution on TLC plate. I want to know the amount of sample should be loaded on plated for detection. Thanks.

31 December 2017 2,895 7 View

How I can separate glycosphingolipids from total cell lipid?

I am trying to extract glycosphingolipid from total cellular lipids. Later I will detect shingolipid by HPLC. Thanks

07 August 2017 6,108 4 View

Would you help to extract Globotriaosylceramide (GB3) from cell?

06 July 2017 5,174 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View