Kartik R. Roy

15 Questions 13 Answers 0 Followers

Questions related from Kartik R. Roy

Is there any glycoshpingolipid detection technique based on HPLC or UV?

I want to detect glycosphingolipid by UV detector in HPLC. Please, anyone let me know.

11 November 2018 9,508 2 View

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

09 September 2018 8,125 6 View

How can I measure Glucosylceramide or lactosylceramide by HPLC or spectrometer?

I want to measure glucosylceramide or lactosylceramide quantity by spectrometer or HPLC.

04 April 2018 2,082 4 View

How can I get a gene ontology software for free?

I need to download gene ontology software for free. Thanks

03 March 2018 3,144 0 View

Why double band appeared on TLC plate?

I run standard mixture on TLC plate. All standards showed with double band.

02 February 2018 3,177 4 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

02 February 2018 2,690 3 View

How can I download protein structure having mutation hotspot mark?

I want to download p53 protein structure with hotspot mutation mark from protein data bank. But I could not. Would you suggest me how to use PDB or other website to see protein structure? Thanks

01 January 2018 3,679 1 View

How much sample (in amount) should be loaded on TLC plate?

I am loading 10 microliter sample slution on TLC plate. I want to know the amount of sample should be loaded on plated for detection. Thanks.

01 January 2018 2,950 7 View

How I can separate glycosphingolipids from total cell lipid?

I am trying to extract glycosphingolipid from total cellular lipids. Later I will detect shingolipid by HPLC. Thanks

08 August 2017 6,146 4 View

Could you tell me cancer resistance cells which express Glucosylceramide synthase.

Glucosylceramide synthase (GCS) performs ceramide glycosylation which is important for resistance to chemotherapy. Which cancer resistance cells express more GCS. Thanks

08 August 2017 9,115 1 View

Would you help to extract Globotriaosylceramide (GB3) from cell?

I am working on cellular sphingolipids. I am looking for extraction of cellular Globotriaosylceramide (GB3) from cell?

07 July 2017 5,216 2 View

What should be ideal antibody amount (primary and secondary) for western blot experiment?

I want to know primary and secondary antibody concentration/amount for western blot experiment. What factors are responsible for determining antibody amount? Thanks

06 June 2017 4,798 4 View

What is the storage (freezing) condition for Mouse Fibroblast Cell?

I am culturing mouse fibroblast cell in 10% FBS medium with 1% PSG and 0.1% Amp B. What is the condition for this cell to freeze in liquid nitrogen?

04 April 2017 6,462 5 View

Could you explain why buffering agent is important for genetically engineered cell line? Why Sodium Bi-carbonate is used in culture medium?

I am working with TP53 cell line which is genetically engineered cell line by CRISPR-Cas9 system. Supplier recommended to use Sodium Bi-carbonate in culture medium.

12 December 2016 6,992 2 View

Anyone working on drug resistance of TP53 cell line?

How many passages usually need to develop drug resistance for this cell line by two drugs? Could you let me know the cell response to these drugs? 1. Cisplatin 2. 5-FU

11 November 2016 250 2 View