Is there any glycoshpingolipid detection technique based on HPLC or UV?

I want to detect glycosphingolipid by UV detector in HPLC. Please, anyone let me know.

10 November 2018 9,464 2 View

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

08 September 2018 8,079 6 View

How can I measure Glucosylceramide or lactosylceramide by HPLC or spectrometer?

I want to measure glucosylceramide or lactosylceramide quantity by spectrometer or HPLC.

03 April 2018 2,002 4 View

How can I get a gene ontology software for free?

I need to download gene ontology software for free. Thanks

02 March 2018 3,102 0 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

01 February 2018 2,643 3 View

How can I download protein structure having mutation hotspot mark?

I want to download p53 protein structure with hotspot mutation mark from protein data bank. But I could not. Would you suggest me how to use PDB or other website to see protein structure? Thanks

31 December 2017 3,639 1 View

How much sample (in amount) should be loaded on TLC plate?

I am loading 10 microliter sample slution on TLC plate. I want to know the amount of sample should be loaded on plated for detection. Thanks.

31 December 2017 2,895 7 View

How I can separate glycosphingolipids from total cell lipid?

I am trying to extract glycosphingolipid from total cellular lipids. Later I will detect shingolipid by HPLC. Thanks

07 August 2017 6,108 4 View

Could you tell me cancer resistance cells which express Glucosylceramide synthase.

Glucosylceramide synthase (GCS) performs ceramide glycosylation which is important for resistance to chemotherapy. Which cancer resistance cells express more GCS. Thanks

07 August 2017 9,076 1 View

Would you help to extract Globotriaosylceramide (GB3) from cell?

06 July 2017 5,174 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View