Why double band appeared on TLC plate?

More Kartik R. Roy's questions See All

Is there any glycoshpingolipid detection technique based on HPLC or UV?

I want to detect glycosphingolipid by UV detector in HPLC. Please, anyone let me know.

10 November 2018 9,524 2 View

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

08 September 2018 8,141 6 View

How can I measure Glucosylceramide or lactosylceramide by HPLC or spectrometer?

I want to measure glucosylceramide or lactosylceramide quantity by spectrometer or HPLC.

03 April 2018 2,102 4 View

How can I get a gene ontology software for free?

I need to download gene ontology software for free. Thanks

02 March 2018 3,159 0 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

01 February 2018 2,707 3 View

How can I download protein structure having mutation hotspot mark?

I want to download p53 protein structure with hotspot mutation mark from protein data bank. But I could not. Would you suggest me how to use PDB or other website to see protein structure? Thanks

31 December 2017 3,690 1 View

How much sample (in amount) should be loaded on TLC plate?

I am loading 10 microliter sample slution on TLC plate. I want to know the amount of sample should be loaded on plated for detection. Thanks.

31 December 2017 2,963 7 View

How I can separate glycosphingolipids from total cell lipid?

I am trying to extract glycosphingolipid from total cellular lipids. Later I will detect shingolipid by HPLC. Thanks

07 August 2017 6,165 4 View

Could you tell me cancer resistance cells which express Glucosylceramide synthase.

Glucosylceramide synthase (GCS) performs ceramide glycosylation which is important for resistance to chemotherapy. Which cancer resistance cells express more GCS. Thanks

07 August 2017 9,128 1 View

Would you help to extract Globotriaosylceramide (GB3) from cell?

I am working on cellular sphingolipids. I am looking for extraction of cellular Globotriaosylceramide (GB3) from cell?

06 July 2017 5,229 2 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

Md Sahadat Hossain Popular answer

Well side @Tanwir Athar

Gunawan Indrayanto

Hi, Did you have pure standard? Did you mean that your standard appeared as 2 bands (or two spots)? Please check the spectra UV/VIS of the two bands, check also the purity of those two bands. It could be your standard was not pure compound.

Hendy Suhendy

- Make sure the purity of the standard compund using 2D TLC

- Check also contaminant compund on the solvent

Md Sahadat Hossain