I want to detect glycosphingolipid by UV detector in HPLC. Please, anyone let me know.
Dear Sir. Concerning your issue about the glycoshpingolipid detection technique based on HPLC or UV. I think the following below links may help you in your analysis:
http://www.cyberlipid.org/ceret/cere0004.htm
https://pubs.acs.org/doi/pdf/10.1021/bk-1980-0128.ch001 http://lipidlibrary.aocs.org/Analysis/content.cfm?ItemNumber=40372
Thanks
Terminology: "UV" is a detection mode. "HPLC" is an analytical technique for the separation of compounds.
Yes, you can certainly use HPLC coupled with an appropriate detector for the analysis of glycosphingolipids. Because they can not be detected in their native form by UV/VIS analysis we use specialized detectors which do not rely on light transmission properties alone. For example, you can use Refractive Index detection (RID), Evaporative Light-Scattering Detection (ELSD) or Mass spectrometry (to name a few).
If you derivatize the compounds in their natural form, then UV detection may be used as they do not have a strong enough chromophore to be detected without modification. If you derivatize the samples, you may also be able to use other analytical techniques such as GC too. BTW: Derivatization should always be your last choice as it may introduce error into your analysis. Sometimes a lack of suitable alternative methods, knowledge or instruments leaves you with no other choice.
Next time you have a very basic question like this one, please try a key-word search on the web first. You will learn HOW to research a question and advance your own knowledge in the process.
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