Which medium must we use for culturing of human microglial HMC3 cell line?

Hi everyone, We will use human microglial HMC3 cell line in our experiments. It's written in ATCC that EMEM medium must be used for culturing but DMEM/F-12 medium was used in some articles. So,...

27 November 2018 7,498 4 View

What is the best tool to reveal the sequence of the mature form of an unknown miRNA using bioinformatics?

Unfortunately, existing miRNA databases are of no help... Any suggestions? Thanks.

18 February 2015 2,306 4 View

Do you coat petri dishes with 2% gelatin (Sigma G1393) when growing HUVEC-C (ATCC CRL-1730)?

Do you coat petri dishes with 2% gelatin (Sigma G1393) when growing HUVEC-C (ATCC CRL-1730)?

15 November 2014 9,601 3 View

The Learning Factory for Industry 4.0

Can the Learning Factories (LF) method used in education be used to increase awareness and professional skills of employees and business owners in enterprises for Industry 4.0?

01 January 1970 9,950 14 View

Climate Change and Green SMEs

Climate change continues to have a rapid impact in the world. Countries are taking a series of measures to combat climate change. At this point, the environmental impacts of SMEs should not be...

01 January 1970 9,439 3 View

Which qualification is required for the digital entrepreneur?

The way of development in the economic, social and cultural fields in the information society passes through the combination of qualified person, qualified enterprise, qualified society and even...

01 January 1970 8,933 9 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View