Does anybody know about antibody production by public research facilities?

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Can anyone suggest why the U87-MG glioblastoma cell line did not attach after thawing?

The cells do not confluency after thawing. Even after 10 days, the color of the medium does not change. I change the medium, the non-adherent cells disappear and then the density in the flask...

11 December 2023 7,002 2 View

Which commercial extrinsic fluorescent dyes can we use to monitor protein unfolding & aggregation?

Hi All, In our lab, we use ThT, ANS, and Bis-ANS dyes to analyze protein characteristics after unfolding. Now, we are planning on obtaining new extrinsic fluorescent dyes. We have a list...

10 September 2022 3,448 0 View

What kind of parameters (or metabolites) can I detect to examine toxicity/alterations in cells?

Hi All, I'm measuring cell toxicity in different cell lines against aggregated protein solutions. I'm performing various cell viability, toxicity, and ROS level assays. Except for these, what...

20 November 2021 7,684 3 View

Why do I struggle to adjust/lower pH of my phosphate buffer at a certain pH point, even though I add too much acidic solution?

I was preparing a phosphate buffer and adjusting its pH to 7.2 from 9.0 by adding monobasic phosphate. In the beginning, it started to decrease fast but then, it went slowly (it was about pH=7.6)....

06 September 2021 5,518 3 View

How can I improve this questionnaire?

How can I improve this questionnaire? How many dimensions should I devote to the questions prepared to raise awareness in enterprises? Should I do the sizing process with the factor analysis...

12 December 2018 6,078 10 View

Which medium must we use for culturing of human microglial HMC3 cell line?

Hi everyone, We will use human microglial HMC3 cell line in our experiments. It's written in ATCC that EMEM medium must be used for culturing but DMEM/F-12 medium was used in some articles. So,...

27 November 2018 7,697 4 View

What is the best tool to reveal the sequence of the mature form of an unknown miRNA using bioinformatics?

Unfortunately, existing miRNA databases are of no help... Any suggestions? Thanks.

18 February 2015 2,424 4 View

Do you coat petri dishes with 2% gelatin (Sigma G1393) when growing HUVEC-C (ATCC CRL-1730)?

15 November 2014 9,722 3 View

Could someone provide us with a working chicken egg yolk tumor xenograft test protocol?

Tumor xenograft tests in chicken egg yolk. What are the pitfalls? Thanks

04 May 2014 4,374 3 View

The Learning Factory for Industry 4.0

Can the Learning Factories (LF) method used in education be used to increase awareness and professional skills of employees and business owners in enterprises for Industry 4.0?

01 January 1970 10,061 14 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What's biological importance of bacteria &role of microbes improve crop productivity &soil health &role of bacteria as decomposers incycling nutrient?

What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?

31 July 2024 3,479 5 View