Does anybody know about antibody production by public research facilities?

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Which medium must we use for culturing of human microglial HMC3 cell line?

Hi everyone, We will use human microglial HMC3 cell line in our experiments. It's written in ATCC that EMEM medium must be used for culturing but DMEM/F-12 medium was used in some articles. So,...

27 November 2018 7,498 4 View

What is the best tool to reveal the sequence of the mature form of an unknown miRNA using bioinformatics?

Unfortunately, existing miRNA databases are of no help... Any suggestions? Thanks.

18 February 2015 2,306 4 View

Do you coat petri dishes with 2% gelatin (Sigma G1393) when growing HUVEC-C (ATCC CRL-1730)?

15 November 2014 9,601 3 View

Could someone provide us with a working chicken egg yolk tumor xenograft test protocol?

Tumor xenograft tests in chicken egg yolk. What are the pitfalls? Thanks

04 May 2014 4,264 3 View

The Learning Factory for Industry 4.0

Can the Learning Factories (LF) method used in education be used to increase awareness and professional skills of employees and business owners in enterprises for Industry 4.0?

01 January 1970 9,950 14 View

Climate Change and Green SMEs

Climate change continues to have a rapid impact in the world. Countries are taking a series of measures to combat climate change. At this point, the environmental impacts of SMEs should not be...

01 January 1970 9,439 3 View

Which qualification is required for the digital entrepreneur?

The way of development in the economic, social and cultural fields in the information society passes through the combination of qualified person, qualified enterprise, qualified society and even...

01 January 1970 8,933 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View