How many generations of hybrid rice should I grow to obtain true breeding lines?

I crossed two lines of rice with contrasting leaf, culm and panicle traits. The F1 plants were almost halfway between the parents except for some quantitative heterosis. The F2 plants (currently...

08 September 2018 7,703 8 View

How carefully or how carelessly do editors of some journals handle our manuscripts?

On 24 August 2017, I submitted a manuscript to A JOURNAL WITH AN IMPACT FACTOR OF MORE THAN 2.0. Four months later, the manuscript status was under review. I felt this was enough for the journal...

02 March 2018 6,694 1 View

How to decide on buying an HPLC system?

09 October 2016 6,549 8 View

Is it sensible to cite a paper that is published in a language other than English?

I do not speak Spanish or Japanese and defenitley many researchers do not speak arabic. Is it acceptable and sensible to cite a paper that was originally published in a language other than...

08 September 2015 7,788 3 View

Does someone have pTOK233 or pSB134?

Does any colleague have pTOK233 or pSB134 and want to share?

01 February 2015 5,766 1 View

Why does not the barrier to radial oxygen loss in rice root limit water permeation?

When rice is grown in anaerobic soil, it develops barrier to radial oxygen from root to soil. This barrier is mainly suberin in the outer layers of root. Is it logical that this barrier does not...

08 September 2014 1,667 5 View

Could anyone suggest a protocol for date palm propagation, based on lab experience not just theoretically?

Date palm micropropagation

03 April 2014 5,082 0 View

If the same job is done with real time RT-PCR, will it be able to discriminate between products of specific reaction and other unintended products?

To quantify expression of a gene with semi-quantitative RT-PCR, the cDNA is synthesized and equalized based on an internal control gene such as 18S rRNA. The gene of interest is then amplified...

02 March 2014 472 3 View

Is it fair to consider the rate of transpiration and stomatal conductance as measures of combined root and shoot hydraulic conductance?

Hydraulic conductance

01 February 2014 4,477 20 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View