Hi everyone,
I am planning to use TGF-β to transform my fibroblasts into CAFs. Most of the papers I have been reading have used TGF-β from R&D Systems, but none of them has mentioned whether they have used the one with or without carrier. Their website recommends TGF-β with carrier for cell culture purposes, but I'm not sure why is that and how using the wrong one can affect the results of my experiment. Any suggestions?
Thanks!
Hello
The carrier is none other than BSA. TGF-beta with carrier will enhance protein stability and increase shelf life. So TGF-beta with carrier is recommended for cell culture application and as a standard in ELISA. It is not recommended in those applications where BSA cause interference and so in such cases TGF-beta without carrier is recommended.
So in your case you can use TGF-beta with carrier. It will not affect your results in anyway.
I hope this is helpful.
Best Wishes.
R&D Systems has professional and efficient technical support scientists. I had some good experience with them. Drop them a message or just ring them, they can give you prompt reply.
Thanks so much for your help, I ended up ordering the one with carrier and I hope it doesn't take too long to be delivered.
Malcolm Nobre and Shiqiu Xiong
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