Hi
I used to make self made SDS-PAGE gels and stained them with coomassieblue (coomassieblue 0.05%, methanol 40%, H2O 50%, acetic acid 10%) with a destaining step afterwards. sometimes I STAINED overnight and destained for several hours. both staining overnigt or destaining overnight worked fine and I got visible bands.
now I have changed to Mini-PROTEAN® TGX™ Precast Gels http://www.bio-rad.com/en-ch/product/mini-protean-precast-gels/mini-protean-tgx-precast-gels. I used the same staining solution, overnight, and destained the day after. No band was visible/stained, even some bands of the ladder were lost.
what could be the problem?
I have already asked Biorad tech support without getting any useful answer
thanks
Hi, thanks
I STAINED Overnight. And destained afterwards. Before destaining was complete it was already obvious that staining had not worked and before I destained already bands of the prestained ladder had been lost. So I do not think that destaining was a problem but rather the staining. There must be an obvious difference between the gels.
TGX gels don't contain SDS according to Biorad (read their info), and there are probably other differences to a typical SDS-PAGE gel aswell.
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