Does anyone know good kit for isolation RNA from fatty tissue, like brain? Thx for help!
We isolated RNA from brain with TRIreagent (or Trizol) following chloroform/isopropanol/ethanol, everything went ok. Good luck!
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How to explain these figures?
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I'm using a solution of graphene oxide quantum dots (GOD) to absorb the energy from a visible light source. The bandgap of GOD is 2.3 eV, the light source intensity is 40W/cm2. So how can I know...
19 June 2019 5,092 3 View
Hi I used to make self made SDS-PAGE gels and stained them with coomassieblue (coomassieblue 0.05%, methanol 40%, H2O 50%, acetic acid 10%) with a destaining step afterwards. sometimes I STAINED...
26 October 2017 7,377 3 View
Hi, Am trying to separate OmpF and C of some E.coli on SDS PAGE. using the ORFs of these proteins from WGS estimate protein sizes of approx 40 and 42kDa. I do precipitate my protein preps before...
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I am doing immunohistochemistry (IHC) in Prefrontal cortex of brain section slices after fixing by formalin 4%. My IHC images have so many white vacuole, has anyone who has experienced this...
30 April 2017 9,891 7 View
I'm using pCP20 vector carrying gene for FLP recombinase to remove kanamycin resistance cassette from bacteria genome. I know that there are two version of this vector: temperature or L-arabinose...
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05 January 2015 1,269 10 View
Are we at risk of further development of the remote labor market? Will it affect the development of the precariat?
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How to calculate the RMSD values for a MD simulation using MOE?
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When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
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The role of climate change influencing the human behavior as well as economics interest
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