I am facing the severe bacterial contamination after 2 to 15 days of sub-culturing of sugarcane as this is noticed as yellowing of leaf in attached photo. Can anyone help me how can I save these plants. Photo is attached below
Dear Sapna Yadav it seems has a difficult solution. Besides of weakeness of plants, which wouldn't allow an acute disinfection, the high contamination degree makes almost impossible tpo save them. Anyway, you could try the culture in media with antibiotics: Cefotaxime and/ Carbenicillin. But you must act as soon as possible. Select the less affected materials, and try with them. Good luck
Well, it's a bit of a hard decision here. Your plants are not doing well and it is going to be challenging to save them in tissue culture. If what you really need are live plants you could try moving them to soil. You'd have to start over for subculturing but could at least try to save these ones. I agree with adding some broad spectrum antiobiotics to the culture medium.