Parisa Faraji

4 Questions 3 Answers 0 Followers

Questions related from Parisa Faraji

Co-treatment of Rsl3 and Ferrostatin-1?

Hello to all, I am working on SH-SY5Y cells and I treated my cells with Rsl3 and ferostatin-1 (24 hours) but I could not see good cell viability. I would like to know how I can treat my cell so...

30 July 2023 7,716 2 View

SH-SY5Y cells seeding and Western blot and q-pcr?

Hi, I have some problems with SH-SY5Y cells. I seeded 20000 cells/well in a 96-well plate and had no problem, but now I have a problem in seeding my cells in a 6-well plate. I seeded different...

15 May 2023 3,284 3 View

How to measure GSH amount in Hippocampus?

I homogenize my sample with PBS and then I add solphosalcylic acid %4 in same mount in the supernatant. The sample incubated for 30min in 4c and then centrifuged in 4000g for 10min and I add...

07 May 2022 441 2 View

Does anybody know how to clone ApoE4 gene?

There are five transcript variants and two isoforms of ApoE. I am not sure about the sequence of ApoE4 cDNA.

26 November 2019 2,241 3 View