Chondrogenesis of MSCs - why there is no difference in the chondrogenic ability of Tgfb1 and Tgfb3?

More Subhash C. Juneja's questions See All

In transient simulations, Is it necessary that the solution to be converged within the given max iterations per time step?

I am doing Large eddy simulation in ansys fluent. Currently i am using unstructured mesh, so the grid size to compute the CFL number is not fixed, Is it necessary that the solution to be converged...

30 November 2023 7,889 4 View

Please suggest dyes for fluorescent labelling of proteins lacking Cysteines and Lysines?

Hello, I am looking for suggestions for labeling proteins lacking any cysteins and lysines. While, SDS mutation to add a cysteine or lysine are possible options. I am looking for suggestions...

29 November 2023 7,740 1 View

How to make sample for ELISA measurement of cytosolic pro-IL3 beta?

Help will be appreciated.

25 October 2023 6,797 0 View

How to calculate expected percentage mortality of synergy experiment in insect bioassay?

combination of two toxins

28 June 2023 2,996 1 View

Which publications of mine are weighted?

Kindly do needful

15 June 2023 904 0 View

What is the pH of buffalo oviductal fluid (NOT cattle)?

If you have any research article which directly saying pH of oviductal fluid in buffalo, Please share it with me. I really appreciate any help you can provide.

14 May 2023 3,656 5 View

What are available mouse models of glaucoma?

What are best models in mouse to study glaucoma? Is intracameral injections of beads a good model?

25 April 2023 812 0 View

Does selenium is essential nutrient for plant growth and development or not ?

What are most physiological aspect of essential nutrient as Selenium .Which crop is most sensitive for it. Kindly highlight in term of research and deployment.

18 April 2023 1,005 2 View

How to enter entering in C1000 Biorad thermal cycler for touchdown step of PCR program?

Hi I want to program C1000 for touchdown PCR protocol (pl see enclosed file) If someone can help me how to enter it in C1000 machine Thanks

04 February 2023 2,233 0 View

What are the cost of LCA softwares SimaPro and GaBi with databases?

I need the price for SimaPro and GaBi LCA softwares for writing a proposal. If any one have these recent prices or some sources please let me know. Thank you for your help soon.

29 August 2022 3,232 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Charan Thej

Dear Subash,

TGF-beta-1 is mostly related to fibrosis and wound healing and TGF-beta-3 is mostly used for chondrogenic induction and yes TGF-beta-1 has also been used for chondrogenic induction by a few groups.

TGF-beta family functions are widespread in their areas of development and function.

I am attaching a brilliant article published in Cell Stem Cell.Take a look.

Hope this helps

Subhash C. Juneja

Dear Charan,

Thanks a lot for the reply, I am very happy that you supported me on this issue. I will read this article by Elaine Fuchs group. This will clarify my thoughts on Tgfbs.

Best,

Subhash