I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

Is there a book that is of great benefit to a new research scholar working in the supply chain model?

I am talking about supply chain, so that the new research scholar will be of great benefit. After reading that, he was able to do a lot of improvement in his research.

31 December 2020 933 4 View

When we submit the paper, we have to give the names of the referee while submitting the paper in the journal. So what is the effect on the paper?

During the paper submission

22 December 2020 5,788 4 View

Can anybody help to find a tool for assessing study habits of students?

It is for my research purpose

21 December 2020 833 3 View

How I can choose best response surface methodology design for optimisation of of PH, temperature, metal ion concentration, adsorbent dose?

I wanted to use CCD aur box-behnken design Which one is better and how I can do experiment using design expert software

13 December 2020 6,323 5 View

Can i use Lactose instead of IPTG for M9 protein expression?

Hello all, Is it possible to use lactose instead of IPTG for M9 protein expression? Glucose is traditionally used as the carbon source for labelling proteins and we used 2% final in the media....

14 October 2020 3,584 4 View

Bio-intensive cropping systems can reduce the environmental foot print?

The adoption of bio-intensive cropping systems has the potential to reduce environmental footprints without compromising the economic gain per unit of resources.

12 October 2020 2,625 7 View

Application of Polyhalite changes the nutrients chemistry in soil?

Polyhalite is a multi-nutrient source, its application assume to be changes the soil biology and nutrients chemistry on long term basis

12 October 2020 6,685 3 View

How to store cell lysate for MDA assay?

Hi, I am working on HK-2 cells and want to check for MDA accumulation after treatment with our compounds of interest. I am using MDA assay kit from Sigma. However, the kit does not mention about...

03 September 2020 1,795 2 View

Do you want to learn more about One Health Biorisk Management?

An Invitation For the global health security, developing a One Health Biorisk Management (BRM) community of practice is essential. In this line, Vietnam One Health University Network (VOHUN) in...

28 August 2020 3,730 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

How to measure the pH of Peat Soil?

Peat possess organic content above 75% and is highly acidic in nature (i.e. pH < 4). Before commencing stabilization, it is mandatory to know about the pH level of peat. Therefore, your...

18 February 2021 9,424 7 View