I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

Is there a book that is of great benefit to a new research scholar working in the supply chain model?

I am talking about supply chain, so that the new research scholar will be of great benefit. After reading that, he was able to do a lot of improvement in his research.

31 December 2020 933 4 View

When we submit the paper, we have to give the names of the referee while submitting the paper in the journal. So what is the effect on the paper?

During the paper submission

22 December 2020 5,788 4 View

Can anybody help to find a tool for assessing study habits of students?

It is for my research purpose

21 December 2020 833 3 View

How I can choose best response surface methodology design for optimisation of of PH, temperature, metal ion concentration, adsorbent dose?

I wanted to use CCD aur box-behnken design Which one is better and how I can do experiment using design expert software

13 December 2020 6,323 5 View

Can i use Lactose instead of IPTG for M9 protein expression?

Hello all, Is it possible to use lactose instead of IPTG for M9 protein expression? Glucose is traditionally used as the carbon source for labelling proteins and we used 2% final in the media....

14 October 2020 3,584 4 View

Bio-intensive cropping systems can reduce the environmental foot print?

The adoption of bio-intensive cropping systems has the potential to reduce environmental footprints without compromising the economic gain per unit of resources.

12 October 2020 2,625 7 View

Application of Polyhalite changes the nutrients chemistry in soil?

Polyhalite is a multi-nutrient source, its application assume to be changes the soil biology and nutrients chemistry on long term basis

12 October 2020 6,685 3 View

How to store cell lysate for MDA assay?

Hi, I am working on HK-2 cells and want to check for MDA accumulation after treatment with our compounds of interest. I am using MDA assay kit from Sigma. However, the kit does not mention about...

03 September 2020 1,795 2 View

Do you want to learn more about One Health Biorisk Management?

An Invitation For the global health security, developing a One Health Biorisk Management (BRM) community of practice is essential. In this line, Vietnam One Health University Network (VOHUN) in...

28 August 2020 3,730 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View