06 April 2013 1 2K Report

I would like to perform zymography of partially purified chitinase sample. Can anyone suggest a zymography procedure for chitinase without incorporating glycol chitin in the gel?

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How can I calculate the empirical orthogonal function?

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Can we re-incubate the western blot membrane for the same protein with same primary and secondary antibody?

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27 May 2023 1,127 3 View

What are the growth characteristics of mammalian cells? Is the mammalian growth curve same as bacterial growth curve?

I am working recombinant protein production using Chinese Hamster Ovarian cells. Need to understand the growth pattern of mammalian cells and how the metabolism rate affects the recombinant...

28 November 2022 8,731 3 View

Log and normal scales equation interpretation ?

Hello all I'm interested in deducing a graph I have two values, one increases exponentially which is taken on X axis and other value taken on y axis increases normally (normal scale) taken. Can I...

17 December 2020 8,480 2 View

Association between categorical and continuous variable?

Hey all researchers, I've a little query, how can one find the association between the nominal (say as case & control, dependent variable) and different continuous variables (independent...

14 October 2019 6,258 9 View

How much sequence coverage is needed for global DNA methylation analysis by bisulphite conversion and sequencing of LINE1 gene?

I am trying to understand the levels of methylation using LINE1 gene (surrogate marker for global methylation) in mice. My aim is to see the levels of methylation between control and test, hence i...

11 July 2019 7,043 1 View

How to separate low molecular wt protein on SDS-PAGE?

Molecular weight of my protein of interest is 7KDa. I am not able to get this on 18% SDS-PAGE electrophoresis. I want to do western blotting of my samples having this. Kindly suggest the method to...

19 March 2019 3,064 9 View

How to study the global DNA methylation?

I am working with DNA methylation currently. I am creating hypomethylation condition in the cells. so how to confirm that the hypomethylation has occurred. sequencing of only LINE-1 gene will be...

19 March 2019 3,368 1 View

Ideally, how many internal controls should be used for Real time PCR? which are the commonly used internal controls to study mice oocytes and embryos?

I am working with embryos and oocytes, i am confused about how many an which internal controls to use for qPCR? in literature, i have seen only one internal control has been used and study is...

28 February 2019 5,760 1 View

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