I am trying to understand the levels of methylation using LINE1 gene (surrogate marker for global methylation) in mice. My aim is to see the levels of methylation between control and test, hence i am doing bisulphite conversion followed by pyrosequencing of LINE1 gene.

Here, i am confused about sequence coverage.

LINE1 gene is 1.4kb, can anyone suggest me how much sequence i have to cover during sequencing so as to see overall methylation level?

More Riddhi Kirit Pandya's questions See All
Similar questions and discussions