How much sequence coverage is needed for global DNA methylation analysis by bisulphite conversion and sequencing of LINE1 gene?

I am trying to understand the levels of methylation using LINE1 gene (surrogate marker for global methylation) in mice. My aim is to see the levels of methylation between control and test, hence i...

11 July 2019 6,893 1 View

Why is there so much peak broadening in MALDI for my protein sample?

There are two samples MBP1 and MBP2 which are same samples of high concentration of Maltose Binding Protein (3-4 mg/ml). The other flatter samples is basically same sample but with 1 mM Hydrogen...

01 May 2019 9,321 3 View

How to separate low molecular wt protein on SDS-PAGE?

Molecular weight of my protein of interest is 7KDa. I am not able to get this on 18% SDS-PAGE electrophoresis. I want to do western blotting of my samples having this. Kindly suggest the method to...

19 March 2019 2,948 9 View

How to study the global DNA methylation?

I am working with DNA methylation currently. I am creating hypomethylation condition in the cells. so how to confirm that the hypomethylation has occurred. sequencing of only LINE-1 gene will be...

19 March 2019 3,213 1 View

Ideally, how many internal controls should be used for Real time PCR? which are the commonly used internal controls to study mice oocytes and embryos?

I am working with embryos and oocytes, i am confused about how many an which internal controls to use for qPCR? in literature, i have seen only one internal control has been used and study is...

28 February 2019 5,618 1 View

Why does addition of Tris buffer at pH 8.0 inhibits the ability of HEPES buffer at pH 8.0 to reduce gold ions?

17 October 2018 5,111 1 View

What exactly is happening to my protein crystals when I peel the tape sealing the well ?

These are Maltose Binding Protein crystals and I have to open the seal to make a buffer transfer. The moment I open the wells, within 2 mins the solution turns opaque as shown. Can someone help me...

03 April 2018 5,479 2 View

Can anybody explain in two-phase air-water adiabatic pipe flow case which solver is more relevant for simulation in OpenFOAM?

Hi everyone! I want to know that for two-phase air-water in pipe flow case, which solver is more relevant for simulating this problem in OpenFOAM for adiabatic conditions? I am using InterDyMFoam...

09 February 2017 2,702 3 View

Can anybody explain the free body diagram for plug or slug type two-phase air-water horizontal pipe flow?

Hello everyone, I am recently working on experimental analysis of intermittent type two-phase air-water horizontal pipe flow. In this regards, i want to eagerly know the dynamics of intermittent...

28 December 2015 3,680 4 View

How to set interface boundary conditions in OpenFOAM?

It is very simple to set interface boundary condition in fluent but I am facing difficulty for giving boundary condition in OpenFOAM. I am trying to simulate the problem of pipe flow in which air...

07 February 2014 7,744 11 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Could you please suggest to me research in which a relation between the shear wave velocity and the degree of saturation for stone was discussed?

Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards

24 February 2021 9,690 1 View

Algorithms to filter corrective max and min from a set of data?

I've got this 5 year Direct Normal Irradiance data I downloaded from https://re.jrc.ec.europa.eu/. I ordered this by year and by every 24 hours because I need the DNI curve by max in summer, min...

17 February 2021 2,777 5 View

There is any numerical criterion for identifying neighbours for Voronoi /Gabriel graphs?

If there is a set of n points in a plan (with known x, y coordinates), can I use a numerical criterion to establish if two points are neighbours, given the definition of a neighbour for Voronoi...

17 February 2021 6,914 4 View

Can anyone name this graph type?

I've attached a recreation of this graph type that I need to make for study reports. The clients have sent them to us in the past, but I am trying to make the reporting process more streamlined,...

15 February 2021 9,795 3 View