which neuronal cell line is RELATIVELY the cheapest (in terms of cell price itself+medium+additives) and easiest to handle and culture?
But still representative to study the interaction between biomaterials and neuronal cells?
Hey Gloria, it depends on the degree of maturity of the neuronal cells you wish to investigate. Is the species, or the origin (CNS or PNS) critical? If not you can try Neuro2A (murine), PC12 (rat PNS) or SH-SY-5Y (human). All of them are easy to handle and have no special medium requirements. However- neuronal cell lines are never identical with mature neurons also concerning molecules critical for interactions with biomaterials such as NCAMs and so on.
Alternatively, you can try to use commercially avaiable neuronal progenitors and differentiate them towards neuronal differentiation - this approach is more promising, but not that cheap (growth factors, e.g. FGF2 and EGF and supplements such as B27 and N2).
Good luck,
Darius
Hey Gloria, it depends on the degree of maturity of the neuronal cells you wish to investigate. Is the species, or the origin (CNS or PNS) critical? If not you can try Neuro2A (murine), PC12 (rat PNS) or SH-SY-5Y (human). All of them are easy to handle and have no special medium requirements. However- neuronal cell lines are never identical with mature neurons also concerning molecules critical for interactions with biomaterials such as NCAMs and so on.
Alternatively, you can try to use commercially avaiable neuronal progenitors and differentiate them towards neuronal differentiation - this approach is more promising, but not that cheap (growth factors, e.g. FGF2 and EGF and supplements such as B27 and N2).
Good luck,
Darius
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