Cell permeable dye to visualise GPCR signalling?

15 February 2019 0 5K Report

I want to perform live cell imaging of GPCR signalling pathways and need a cell permeable dye-conjugate that will show the pathway is active, without affecting downstream signalling, preferably by binding to activated G alpha proteins. I can't use FRET because I need to study the signalling in primary samples.

Thus far, I haven't been able to find anything suitable. Does anyone know of such a dye-conjugate that I might be able to use?

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