Martin Kenny

3 Questions 1 Answers 0 Followers

Questions related from Martin Kenny

How to make PDMS bio-compatible after silanization?

I am trying to examine cell morphology on flat PDMS structures using negative molds also made of PDMS. The negative molds are activated by oxygen plasma treatment and silanized by vapor deposition...

22 November 2019 6,858 1 View

BsmbI digestion not working?

I'm using BsmbI from NEB to digest a CRISPR lentiviral plasmid so I can insert my gRNA sequence. The digestion isn't working at all despite extending the reaction time to 1.5 hours. Other answers...

09 November 2017 8,778 1 View

How can I identify a pure neutrophil population in whole blood by flow cytometry?

I want to examine neutrophil populations in whole blood. I've read that dot plots of CD45 and SSC can be used to gate for granulocytes, but I need to exclude basophils and eosinophils from my...

02 December 2016 4,767 6 View