Cell lines related with receptors, enzymes etc? - FAQS.TIPS

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How can I find a dataset of pathological EMG signals ?

The importance of disease classification studies for the development of diagnosis & prognosis systems is crucial. Here the main problem is the lack of available medical datasets to use. For...

31 October 2023 1,151 4 View

Why AF488 anti-human FoxP3 antibodies are used in Treg staining kits instead of FITC conjugated?

Dear all, I realized that Biolegend has all its Treg staining kits as AF488 conjugated FoxP3 and other dyes for CD25/CD4. What is the reason behind it that they are changing CD25 and CD4...

12 August 2021 3,568 2 View

How important are Cas numbers to the experimental design?

When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6. Then we realize that we don't have enough money...

06 June 2021 3,440 3 View

Is this vector can be used as sgRNA vector?

Hello, I'm trying to make sgRNA plasmid vector for dCas9-VPR- AAV transducted system. In this vector that i shared in below, after screening tag, MCS is presented. I want to use these sites for...

07 October 2020 9,563 0 View

After we co-transfected our hek293 packaging cells for AAV can we stock some of them for future experiments with using freezing & thawing?

Hello, After we co-transfected our hek293 packaging cells with RC, pHelper, GOI for AAV production; can we stock some of them for future experiments with using freezing and thawing method? Are...

06 October 2020 3,896 1 View

Which one is the better strategy for AAV transfer plasmids?

Hello, I designed some systems related with double vector (for dCas9 VPR system and sgRNA casette). I wonder which one is better while applying them and is there any difference between them in...

06 October 2020 1,501 0 View

Is there any AAV serotype that can be transduced to p19 cell line?

Im looking for AAV type for p19 line (for best transduction eff.)

03 October 2020 706 0 View

Do any aav vectors include serotype info?

Hello, I am trying to do new aav2 strategy for dcas9 system. For vector backbones, i use addgene platform. But for aav serotypes, no information were added into addgene vector database, just Aav...

03 October 2020 9,589 3 View

Which AAV serotype is the best for SY5Y and HT22 cells?

I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?

02 October 2020 3,498 2 View

Can be episomal plasmid vector amplified in mitotic cells?

Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?

24 September 2020 1,743 4 View

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Could it be a cell culture contamination?

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How do you delete a duplicate pdf for the same paper on ResearchGate?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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