Cell Line Facility in INDIA

More Sagar Bhowmik's questions See All

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

How can I collect Zirfon membrane for alkaline water electrolysis?

I am doing research in the United Sates America. So, I will pay in US dollar. Could you please tell me how can i get Zirfon membrane? Thank you.

24 June 2024 7,667 0 View

What is the difference between uv visible measurment and phocatalytic degradation measurment?

why uv is done and why photocatalytic measurement is done although both use uv radiation

09 June 2024 8,750 1 View

When can the fitting of the fluorescence anisotropy decays have the negative component ?

it would be helpful if you provide the reference to your answer

11 May 2024 5,989 4 View

Advanced research on ferrites ?

I just completed my M.Sc Physics while researching on ferrites in Photocatalytic property. Now i want to do My Phd does anyone suggest me any topic or any good university for doing my reserch

19 April 2024 3,772 2 View

Does the Primer3 design primers for the COBRA technique? and selecton of single Restriction enzyme for multiple genes?

Hello, RG Members, I have designed the COBRA primers using the UCSC genome browser for the promoter regions of genes of my intreset. are these primers reliable? I have tried the other softwares...

17 April 2024 7,882 0 View

How do I set up two electrode overall electrocatalytic water splitting with temperature control system?

I have a plan to experimental setup for electrocatalytic overall water splitting reaction with temperature control system for studying how much potential required to electrocatalytic water...

02 April 2024 8,136 4 View

Can any one tell me perfect mobile phase with ratio for paper and TLC of sugars?

I'm working on sugars present in honey sample.I tried paper and TLC with different mobile phases like Butanol:Glacial Acetic acid:Water and Butanol:Pyridine:Water but I'm not getting expected...

05 March 2024 9,609 1 View

Can any one tell me how to confirm methane gas production test?

I'm working on utilization of wastes for biogas production.I streak a loopful cowdung slurry collected from biogas plant on sodium thioglycolate media.I got some colonies on the media.I need to...

28 February 2024 1,877 4 View

HOw TO FIND THE RESTRICTION ENZYMES USING rs ID?

Can anyone knows about how to find the restriction sites using rs IDs. Are there any tools to find?

21 February 2024 2,794 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View