Cell cycle analysis?

More Selen Kum Özşengezer's questions See All

Please where can i get data set or can you send me data set for ROA, FinTech services like mobile banking, trading in cryptocurrency, mobile payment ?

Please help me or locate where i can get data set for ROA(Return on Asset) which is the dependent variable , FinTech examples(Independent variables) like mobile banking & digital banking,...

24 April 2023 8,560 2 View

Hello, has anyone used the ImmunoCruz® rabbit ABC Staining System sc-2018 kit before?

It seems like the protocol in the datasheet is missing.Can people using immunohistochemical staining help?

01 March 2023 7,376 1 View

Which statistics method to use in SPSS?

Hi all, I have conducted a research to see the cross-cultural differences for gender bias in recruitment. Data is collected through Qualtrics. Participants were randomly allocated to see either a...

05 August 2019 8,766 7 View

Which methodes are better for the students in univesity education ?

Sgav , L'approach communicative , La methode Classique etc

11 July 2019 5,254 2 View

Could you please help to fill in the survey "Personality, Use of Music and Music Preference"?

Dear all, I am currently doing a project about personality, use of music and music preference. For this project I have posted a survey online: https://forms.gle/dRiFMheRQGg18GzK7 The survey...

02 June 2019 6,962 15 View

Which analysis in SPSS to see the impact of the scores of a questionnaire on candidate choice in the workplace?

Hi everyone, I have conducted a research and have some data of mindfulness scale, it's reliable and valid. Lower total score means people are more mindful, and I want to see its relationship on...

06 April 2019 2,889 3 View

How do I make 0.35 micrometer brain slices stick onto positively charged slides before cresyl violet staining?

I know this somewhat of a stupid question but I wasn't able to figure out the answer after many trials and research. The brain slices are in an anti-freeze solution, so we use Tris-Buffered...

28 August 2018 4,956 4 View

How to measure the variability of a fold change in metabolomics data?

For an experimental design that the control and treatment samples are not paired and where n= 4-5, I wonder what is the best way to measure the variability. My second question is how actually...

22 January 2015 1,524 7 View

Is it possible to have subclones within a bacteria colony?

After a serial passage of plasmid in E coli, I extracted the plasmid from bacteria colonies and sequenced to identify mutations. The peaks were quite nice with almost no background noise but what...

07 March 2014 8,975 8 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Alireza Naderi Sohi

In addition to what Emad Hussein said, using supernatant containing some dead cells and debris would increase the percentage of the subG1 population dramatically. As a result, the percentage of other populations (i.e., G0/G1, S, G2/M) would be abnormally decreased. Hence, any study on cell cycle including the cell cycle arrest will be cloudy.

Selen Kum Özşengezer

Thank you for your helpful feedback.