I know this somewhat of a stupid question but I wasn't able to figure out the answer after many trials and research. The brain slices are in an anti-freeze solution, so we use Tris-Buffered Saline solution to rinse (takes about 30-60 minutes, depending on how many times we need to rinse it) the slices. Then we use either Phosphate-buffered saline or distilled water to put the slices onto the slide (we use charged slides to make sure the slices stick). Later we let the slides dry on a heater. After that we start the defatting portion of cresyl violet staining where we put the slides in distilled water, 70-95-100 percentages of alcohol and xylene. However the slices come off even at the first step of dipping the slides into distilled water (and we're being gentle).
We also tried using floating brain slices but because the brain slices shrink and solidify, we cannot mount them properly. So we need a way to make the brain sliced stick onto slides. Do you have any suggestions on how we should proceed on?
Use a poly-lysine coat on the slides. This promotes better adhesion. I prefer using the precoated ones from VWR. Hope it works!
poly-lysin or gelatin coated slides work well! and we also found out that if we let the slices dry longer they stuck better to the slides :)
Good luck!
We use slides with nourishing gelatin and double chromium treatment, they adhere perfectly and do not detach as with other types of slides. Regards
Yes, I would go with the gelatin coated slide. But before gelatin you should treat the slides with potassium chromium sulphate. Please see a good protocol from the internet. Gelatin only is not very good. The potassium chromium sulphate makes covalent bonds with the gelatin and the glass slides. This will be better than the commercially available slides. I have 10 years of experience in support of this!
Regards
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