Can I continue cell culture with e14t2ga embryonic stem cell lines without the feeder layer, specially in 0.1 to 1% gelatin ?
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
Theoretical method for perovskite solar cell
12 March 2024 9,329 0 View
How to apply shear force of a 3D block .can anyone provide Lammps input script on the application of shear force between two different fixed layer of a block?
31 January 2024 9,025 1 View
Hint: Explore how good Leader-Member Exchange (LMX) can positively impact organizational culture by fostering strong leader-subordinate relationships. Discuss its relevance in the context of Agile...
09 January 2024 8,454 2 View
I have implemented an adsorption test of heavy metal solution using wood straw biochar(without any Acid/Alkali activation) as an adsorbent. The degassing temp was 200C for 6h. After adsorption,...
13 December 2023 5,168 1 View
I am looking for a platform to publish a mini-review, suggestions, please.
22 September 2023 4,392 0 View
I am getting a rate coefficient (Barts-Widom Phenomenological) to be zero for a unimolecular decomposition reaction. Similar reaction with another conformer of the same molecule gives some rate...
04 July 2023 2,963 2 View
I am trying to visualize the change in the molecular geometry along the reaction path using the output from the Polyrate17-C package for a gas phase addition reaction. As mentioned in the Polyrate...
19 June 2023 5,168 0 View
Feel free to help me figuring out.
28 May 2023 4,658 0 View
While computing descriptive statistics should I use means or replications.
16 February 2023 1,165 6 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View