Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Lift off problem with Indium/Gold deposition?

Hi, I'm doing 5/40nm In/Au e-beam evaporation at rates 0.2A/s(for In),1A/s(Au) on lithographically(doubly coated 495k,950k PMMA e-beam resist) done Si substrate at base pressure 2e-6torr. I'm...

18 January 2021 7,550 4 View

How much would be the particle size difference between Malvern Zetasizer Ultra Particle Analyzer and TEM for the same Nanoparticles?

I am measuring the particle size of nanoparticles by Malvern Zetasizer Ultra Particle Analyzer but I am not sure I would get a better size with TEM or not. Because I read in a paper that there is...

05 January 2021 1,612 8 View

Why a distorted admitance value is obtained while doing mixed mode simulation of TFET with dyanamic non local BTBT model in Santaurus TCAD ?

AC analysis of TFET device along with dynamic non local BTBT model shows a distorted admitance. But while using Local BTBT model a good values for the admitance is observed.

20 July 2020 2,465 1 View

If a system is stable in each of the operating regions, can it be said that the system is stable for the entire operating region?

If an operating region is divided into a number of sectors and if it is shown that the system is stable in equilibrium point corresponding to each of the sectors, can it be said that the system is...

21 March 2020 8,816 6 View

Which is the best instrument for the Soil Erosion Measurement?

I want to measure the soil erosion of a watershed. Is there any instrument which will help me to measure the soil erosion?

24 February 2020 8,934 22 View

How to do optimization by neural network in matlab?

I have used 30 experimental data set for ANN modelling (Matlab) and RSM based optimization (Design Expert). The RSM and ANN predicted data was compared and it was observed that the ANN model has...

30 January 2020 7,760 3 View

Where we can find the World geochemical background values of these heavy metals Fe, Zn, Pb, Cr, Cu, Ni, Cd for calc of sediment indexes?

These background values/reference values can be used for calculation of different indexes, viz. enrichment factor, geo-accumulation index, contamination factor, pollution load index and many more.

22 January 2020 8,948 3 View

How to incorporate random uncertainty within the A-matrix represented by a gain block in Simulink?

I have a Simulink gain block that houses the A- matrix of the plant and I want to add randomness into the parameters of A-matrix. How do I achieve that in Simulink?

05 December 2019 6,127 7 View

How to code in Matlab Ellipse by Oblong method?

How to code in Matlab Ellipse by Oblong method? The Video link is given below- https://www.youtube.com/watch?v=TNQE9vN3QJo&t=23s

08 November 2019 3,238 1 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View