Why is it that always the mutant protein is synthesized?

In autosomal dominant disorders I understand that the expression of one allele is required for the proper functioning. In case of mutations why not the protein from the normal allele get...

31 December 2014 4,286 6 View

Protocol for culturing mouse iPSC

Can somebody share protocol for culturing mouse iPSC?

10 November 2014 8,812 3 View

Why is methionine always the first amino acid?

Why does tRNA recognize ATG as the first codon?

05 June 2014 2,240 11 View

Incorporation of ddGTP?

Why does ddGTP gets incorporated faster than other three ddNTPs during sequencing PCR?

04 May 2014 7,427 0 View

Is there any online tool to analyse frameshift mutation?

Is there any online tool to analyse frameshift mutation?

04 May 2014 2,086 6 View

Which is better for GC rich amplification: Betaine or DMSO?

Which is better for GC rich amplification?

03 April 2014 1,772 9 View

What is the difference between physical distance and genetic distance ?

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03 April 2014 9,714 5 View

Why does the gel turn cloudy white as it gets solidified during a Native PAGE protocol?

see above

01 February 2014 9,033 9 View

How do some regions of the gene (or DNA) escape evolution and become conserved?

Thanks in advance.

01 February 2014 5,366 6 View

Can somebody share their GC rich kit protocol for PCR amplfication of difficult templates?

see above

01 February 2014 7,562 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View