check this out

http://www.ncbi.nlm.nih.gov/pubmed/23825368

best,

Ganesh

BLASTX can be used to identify frameshifts by comparing a given nucleotide query with a protein database. :)

http://blast.ncbi.nlm.nih.gov/Blast.cgi

Good way is to translate insilico seq with and without the mutation and compare the giving amino acid sequences for that you can use blasp.

PROVEAN: http://sift.jcvi.org/

and Mutation Taster: http://doro.charite.de/MutationTaster/index.html

I use mutation taster and Varsome.

http://doro.charite.de/MutationTaster/index.html

https://varsome.com/

How can I identify frameshift in my NGS data? I have sequenced 2 tomato lines, and aligned reads against the reference genome. SNPs have been called. Now, I would like an automated method to identify SNPs associated with frameshift, non-synonymous mutations, and or premature stop-codon. Is there any tool?